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中文名称英文名称CAS 编号规格目录价所属品牌订货号
辣根过氧化物酶Peroxidase from horseradish9003-99-010MG580.00SIGMAP6782
辣根过氧化物酶Peroxidase from horseradish9003-99-025MG1,119.00SIGMAP6782
辣根过氧化物酶Peroxidase from horseradish9003-99-0100MG3,259.00SIGMAP6782
品牌SIGMA
产品名称 辣根过氧化物酶 : Peroxidase from horseradish ——【产品编号:SIGMA-P6782】
CAS编号9003-99-0
别名辣根酶,Donor:hydrogen-peroxide oxidoreductase,Horseradish peroxidase
级别科学级
纯度&分析Type VI-A, essentially salt-free, lyophilized powder, 250-330 units/mg solid (using pyrogallol), 950-2000 units/mg solid (using ABTS)
Tips来源于辣根(from horseradish)。
分子式N/A
分子量N/A
储存条件2-8 °C 冷藏保存。
性质
Quality Level PREMIUM
type Type VI-A
form essentially salt-free, lyophilized powder
storage temp. 2-8°C
原产地美国
描述

Analysis Note

The RZ (Reinheitszahl) is the absorbance ratio A403/A275 determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, not enzymatic activity. Even preparations with high RZ may have low enzymatic activity.

This product is assayed using ABTS for easy comparison to other suppliers′ unit: approx. 1,000 units per mg solid

包装

Packaged in mg solid

Unit Definition

One ABTS unit will oxidize 1 μmole of ABTS per minute at 25 °C at pH 5.0

One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

General description

Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.

供货状态True
参考文献

Feng, J., et al., Effects of high temperature on multiple parameters of broilers in vitro and in vivo. Polymer 87, 2133-9, (2008)

Wu, G., and Li, Z., Rapid clearance of circulating protein by early chicken embryo blood cells. J. Immunol. Methods 212, 2176-82, (2009)

Granlund, I., et al., The TL29 protein is lumen located, associated with PSII and not an ascorbate peroxidase. Plant Cell 50, 1898-910, (2009)

Pucker, A.D., et al., Enzymatic Quantification Of Cholesterol And Cholesterol Esters From Silicone Hydrogel Contact Lenses. Invest. Ophthalmol. Vis. Sci. 51, 2949-54, (2010)

类型酶 & 辅酶
安全信息
个人防护装备 Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK德国 3