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中文名称英文名称CAS 编号规格目录价所属品牌订货号
胃蛋白酶Pepsin from porcine gastric mucosa9001-75-6250MG¥680.77SIGMAP6887
胃蛋白酶Pepsin from porcine gastric mucosa9001-75-61G¥1,922.04SIGMAP6887
胃蛋白酶Pepsin from porcine gastric mucosa9001-75-65G¥5,542.87SIGMAP6887
胃蛋白酶Pepsin from porcine gastric mucosa9001-75-610G¥9,585.30SIGMAP6887
胃蛋白酶Pepsin from porcine gastric mucosa9001-75-6100G¥76,213.55SIGMAP6887
品牌SIGMA
产品名称 胃蛋白酶 : Pepsin from porcine gastric mucosa ——【产品编号:SIGMA-P6887】
CAS编号9001-75-6
别名胃蛋白酶 1:10000,Pepsin 1:10000,胃液素,胃蛋白酶 A
级别科学级
纯度&分析lyophilized powder, 3,200-4,500 units/mg protein
Tips来源于猪胃粘膜。
分子式N/A
分子量N/A
储存条件-20 °C 低温冷冻保存。
性质
Quality Level PREMIUM
form lyophilized powder
mol wt mol wt 35 kDa
total impurities salt, essentially free
storage temp. −20°C
原产地美国
描述

Analysis Note

Protein determined by E1%/280

最佳 pH 值为 2-4。在 4M 脲和 3M 盐酸胍中具有活性。在 60°C 下能稳定存在。在 pH 8.0-8.5 下可对胃蛋白酶进行不可逆地灭活。

Application

用于产生抗体的 F(ab′)2 片段。

Unit Definition

One unit will produce a ΔA280 of 0.001 per min at pH 2.0 at 37°C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16 ml. Light path = 1 cm.)

Biochem/physiol Actions

Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin

Pepsin is the major proteolytic enzyme produced in the stomach. It digests proteins through the cleavage of interior peptide linkages.1

供货状态True
参考文献

1. L.R. Johnson, Functional development of the stomach Annu. Rev. Pharmacol., (1985) 47, 199-215

Harlow, E., and Lane, D. Antibodies: A Laboratory Manual Cold Spring Harbor, NY , (1988), 626-628

Lockridge, O., et al., Pseudo-esterase activity of human albumin: slow turnover on tyrosine 411 and stable acetylation of 82 residues including 59 lysines. J. Bacteriol. 283, 22582-90, (2008)

Li, B., et al., Detection Of Adduct On Tyrosine 411 Of Albumin In Humans Poisoned By Dichlorvos. Toxicol. Pathol. 116, 23-31, (2010)

Tako, E., et al., Using The Domestic Chicken (Gallus Gallus) As An In Vivo Model For Iron Bioavailability. Polymer 89, 514-21, (2010)

Ryle, A.P. Meth. Enzym. Anal. 3rd ed., 5, 228, (1984)

Merck 14,7146

类型酶 & 辅酶
安全信息
符号 GHS07GHS08
信号词 Danger
危险声明 H315-H319-H334-H335
警示性声明 P261-P305 + P351 + P338-P342 + P311
个人防护装备 dust mask type N95 (US), Eyeshields, Faceshields, Gloves
危害码 Xn
风险声明 36/37/38-42
安全声明 22-24-26-36/37
WGK德国 1
RTECS SC6132000