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中文名称英文名称CAS 编号规格目录价所属品牌订货号
亮抑酶肽(标准品)Leupeptin hemisulfate salt standard103476-89-72MG1,215.00BSZHSS198
亮抑酶肽(标准品)Leupeptin hemisulfate salt standard103476-89-75MG2,369.00BSZHSS198
品牌BSZH
产品名称 亮抑酶肽(标准品) : Leupeptin hemisulfate salt standard ——【产品编号:BSZH-SS198】
CAS编号103476-89-7
别名亮抑酶酞、亮抑酶肽半硫酸盐,N-乙酰基-L-亮氨酰-L-亮氨酰-L-精氨醛半硫酸盐,亮肽素,Acetyl-Leu-Leu-Arg-al,N-Acetyl-L-leucyl-L-leucyl-L-argininal hemisulfate salt
级别标准品
纯度&分析>99%,Meets standard substance testing specifications
Tips此产品经特殊纯化处理,可用作参照标准、对照等。
分子式C20H38N6O4 · 1/2H2SO4
分子量475.59
储存条件-20 °C 低温冷冻保存。
性质
Quality Level PREMIUM
biological source microbial
assay ≥99% (GC)
solubility H2O: soluble10 mM (Solutions are stable for a week at 4 °C. Stock solutions are stable up to 6 months at −20 °C.)
  H2O: soluble50 mg/mL
storage temp. −20°C
原产地美国
描述

Analysis Note

由于三种形式在溶液中达到平衡,亮抑酶肽可在 HPLC 上形成多个峰。通过三个主峰测定纯度。主要的杂质肽为外消旋亮抑酶肽。

Biochem/physiol Actions

Inhibitor of serine and cysteine proteases. Inhibits plasmin, trypsin, papain, calpain, and cathepsin B. Does not inhibit pepsin, cathepsins A and D, thrombin, or α-chymotrypsin. Effective concentration 10-100 μM. There have been numerous studies using leupeptin to protect against hearing loss caused by acoustic overstimulation or the ototoxic antibiotic gentamicin.1 (Loss of cochlear hair cells is believed to be mediated by calpain.)

供货状态True
参考文献

Berkovich, E., et al., Assessment of protein dynamics and DNA repair following generation of DNA double-strand breaks at defined genomic sites. Nat. Protoc. 3, 915-22, (2008)

Kuznetsov, A.V., et al., Analysis of mitochondrial function in situ in permeabilized muscle fibers, tissues and cells. Nat. Protoc. 3, 965-76, (2008)

Chen, D., et al., Identifying catalytic residues in CPAF, a Chlamydia-secreted protease. Arch. Biochem. Biophys. 485, 16-23, (2009)

Schwarzer, W., et al., A gradient of ROR2 protein stability and membrane localization confers brachydactyly type B or Robinow syndrome phenotypes. Hum. Mutat. 18, 4013-21, (2009)

Zallocchi, M., et al., Biochemical Characterization Of Native Usher Protein Complexes From A Vesicular Subfraction Of Tracheal Epithelial Cells. Biochem. Pharmacol. 49, 1236-47, (2010)

Banaszynski, L.A., et al., Analysis Of Histones And Chromatin In Xenopus Laevis Egg And Oocyte Extracts Methods 51, 40247, (2010)

Aoyagi, T., Inhibitor of trypsin, plasmin, papain and cathepsin B. J. Antibiot. 22, 283, (1969)

Saino, T., et al. Chem. Pharm. Bull. 30, 2319, (1982)

Deutscher, M.P., Maintaining protein stability. Meth. Enzymol. 182, 83-89, (1990)

Jazwinski, S.M., Preparation of extracts from yeast. Meth. Enzymol. 182, 154-174, (1990)

, Beynon, R.J. and Bond, ed. Proteolytic Enzymes: A Practical Approach, (1989), 244-245

类型标准品
安全信息
个人防护装备 Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
安全声明 22-24/25
WGK德国 3