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中文名称英文名称CAS 编号规格目录价所属品牌订货号
乙醇脱氢酶Alcohol Dehydrogenase from Saccharomyces cerevisiae9031-72-57.5KU¥518.00SIGMAA3263
乙醇脱氢酶Alcohol Dehydrogenase from Saccharomyces cerevisiae9031-72-530KU¥1,756.00SIGMAA3263
乙醇脱氢酶Alcohol Dehydrogenase from Saccharomyces cerevisiae9031-72-5150KU¥6,335.00SIGMAA3263
品牌SIGMA
产品名称 乙醇脱氢酶 : Alcohol Dehydrogenase from Saccharomyces cerevisiae ——【产品编号:SIGMA-A3263】
CAS编号9031-72-5
别名ADH,Yeast alcohol dehydrogenase (yADH],乙醇:NAD+ 氧化还原酶 醇脱氢酶 来源于酵母
级别科学级
纯度&分析≥300 units/mg protein
Tips来源于酿酒酵母。
分子式N/A
分子量N/A
储存条件-20 °C 低温冷冻保存。
性质
Related Categories 1.1.x.x Acting on hydroxyl groups, 1.x.x.x Oxidoreductases, Alcohol Metabolism, Application Index, Biochemicals and Reagents, Diagnostic and Analytical Enzymes, Enzyme Class Index, Enzymes, Inhibitors, and Substrates, General Metabolic Enzymes, Metabolomics.
mol wt mol wt ~141,000 (four subunits)
purified by crystallization
suitability suitable for recycling micro-assay of β-NAD and β-NADH
storage temp. −20°C
原产地美国
描述

Unit Definition

One unit will convert 1.0 μmole of ethanol to acetaldehyde per min at pH 8.8 at 25 °C.

Physical form

Solids containing <2% citrate buffer salts.

Application

分子量为 141kDa 的四聚体包含了 4 个相同的亚基。每个亚基的活性位点都含有一个锌原子。每个活性位点还含有 2 个反应性巯基以及一个组氨酸残基。

等电点:5.4-5.8

最适 pH:8.6-9.0

底物:酵母乙醇脱氢酶与乙醇最易反应,它的反应活性随着醇体积增大或减小而降低。与支链醇和仲醇的反应活性也非常低。

KM(乙醇)= 2.1×10-3M
KM(甲醇)= 1.3×10-1M
KM(异丙醇)= 1.4×10-1M

抑制剂:能与游离巯基反应的化合物,包括 N-烷基马来酰亚胺和碘乙酰胺。
锌螯合剂的抑制剂,包括 1,10-菲咯啉、
8-羟喹啉、2,2′-联吡啶以及硫脲。
底物类似物抑制剂,包括 β-NAD 类似物、嘌呤和嘧啶衍生物、氯乙醇以及氟乙醇。

吸光系数:E1% = 14.6(水,280nm)

Biochem/physiol Actions

Alcohol dehydrogenase catalyzes the reversible oxidation of alcohol to acetaldehyde.1

Yeast alcohol dehydrogenase (yADH) serves as an excellent model system for enzyme-catalyzed H transfer because unlike many other enzymes, the chemical step, oxidation of a primary alcohol to an aldehyde by NAD+, is rate-limiting with aromatic substrates.

Specifications

The dried enzyme has been stored for several weeks in a vacuum desiccator with little loss in activity. According to experiments described by A. Kornberg,3 the enzyme can be stored in the frozen state and can be thawed repeatedly without marked loss of activity.

The dried enzyme has been stored for several weeks in a vacuum desiccator with little loss in activity. According to experiments described by A. Kornberg,3 the enzyme can be stored in the frozen state and can be thawed repeatedly without marked loss of activity.1

供货状态True
参考文献

1. E. Racker, Crystalline Alcohol Dehydrogenase from Bakers' Yeast J. Biol. Chem. 184, 313-320, (1950)

Bernofsky, C. and Swan, M. Anal. Biochem. 53, 452, (1973)

Grimm, C., et al., A Crystallization Screen Based On Alternative Polymeric Precipitants. Acta Crystallogr. D 66, 685-97, (2010)

Daniel Roston and Amnon Kohen1, Elusive transition state of alcohol dehydrogenase unveiled Proc. Natl. Acad. Sci. U. S. A. 107, 9572-9577, (2010)

类型酶 & 辅酶
安全信息
个人防护装备 Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK德国 3