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中文名称英文名称CAS 编号规格目录价所属品牌订货号
胰蛋白酶抑制剂Trypsin inhibitor from Glycine max (soybean) 9035-81-825MG472.00SIGMAT6522
胰蛋白酶抑制剂Trypsin inhibitor from Glycine max (soybean) 9035-81-8100MG1,140.00SIGMAT6522
胰蛋白酶抑制剂Trypsin inhibitor from Glycine max (soybean) 9035-81-81G5,593.00SIGMAT6522
胰蛋白酶抑制剂Trypsin inhibitor from Glycine max (soybean) 9035-81-85G22,930.00SIGMAT6522
品牌SIGMA
产品名称 胰蛋白酶抑制剂 : Trypsin inhibitor from Glycine max (soybean) ——【产品编号:SIGMA-T6522】
CAS编号9035-81-8
别名胰酶抑制剂,SBTI
级别细胞培养级
纯度&分析powder, BioReagent, suitable for cell culture
Tips来源于大豆(from Glycine max (soybean) )。
分子式N/A
分子量N/A
储存条件2-8 °C 冷藏保存。
性质
Quality Level PREMIUM
type Type I-S
product line BioReagent
form powder
activity (One mg will inhibit 1-3 mg of trypin with activity of approx. 10,000 BAEE units per mg protein.)
solubility balanced salt solution: soluble1 mg/mL
  serum-free medium: soluble
suitability suitable for cell culture
storage temp. 2-8°C
原产地美国
描述

Analysis Note

Protein determined by biuret.

Application

After trypsinizing cells, resuspend cells in 1 mL trypsin inhibitor solution (1mg/ml) for every mL of trypsin solution used for dissociation. Centrifuge the cell suspension at 1000 rpm for 5 minutes. A cell pellet should form. Remove as much of the trypsin inhibitor solution as possible and resuspend the pellet in serum-free medium. Culture cells as desired.

Preparation Note

Chromatographically prepared.

 

胰蛋白酶抑制剂可以 10mg/mL 或更高的浓度溶于水和磷酸盐缓冲液。浓度较高时溶液可能变浑浊,呈黄色至琥珀色。

Unit Definition

One trypsin unit = ΔA253 of 0.001 per minute with N−α-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C. Reaction volume = 3.2 mL (1 cm light path).

Biochem/physiol Actions

大豆胰蛋白酶抑制剂可抑制胰蛋白酶活性,而很少抑制胰凝乳蛋白酶和纤溶酶活性。它可以 1:1 的化学计量与胰蛋白酶形成复合物。形成这种复合物后,胰蛋白酶可裂解抑制剂中的精氨酸-异亮氨酸单键。这种复合物的解离可生成改性或天然的抑制剂。在胰蛋白酶结合的最佳 pH 值 (pH 8.0) 下,缔合常数 ≥ 10×108

General description

Soybean trypsin inhibitor exhibits a high degree of sequence homology with cathepsin D inhibitor from potatoes.1

供货状态True
参考文献

1. Mares, M., et al., Primary structure of cathepsin D inhibitor from potatoes and its structure relationship to soybean trypsin inhibitor family FEBS Lett. 25, 94-98, (1989)

Giachino, C., et al., Isolation And Manipulation Of Mammalian Neural Stem Cells In Vitro. Methods Mol. Biol. 482, 143-58, (2009)

Hu, Z., et al., Androgen-induced Rhox Homeobox Genes Modulate The Expression Of AR-regulated Genes. Mol. Cancer Ther. 24, 60-75, (2010)

类型酶 & 辅酶
安全信息
符号 GHS08
信号词 Danger
危险声明 H317-H334
警示性声明 P261-P280-P342 + P311
个人防护装备 Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK德国 3