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中文名称英文名称CAS 编号规格目录价所属品牌订货号
木瓜蛋白酶Papain from papaya latex9001-73-425MG¥376.00SIGMAP4762
木瓜蛋白酶Papain from papaya latex9001-73-4100MG¥992.00SIGMAP4762
木瓜蛋白酶Papain from papaya latex9001-73-41G¥6,850.00SIGMAP4762
木瓜蛋白酶Papain from papaya latex9001-73-45G¥21,700.00SIGMAP4762
品牌SIGMA
产品名称 木瓜蛋白酶 : Papain from papaya latex ——【产品编号:SIGMA-P4762】
CAS编号9001-73-4
别名木瓜酶,papayotin
级别科学级
纯度&分析lyophilized powder, ≥10 units/mg protein
Tips高活性木瓜酶,来源于木瓜乳液(from papaya latex)。
分子式N/A
分子量N/A
储存条件-20 °C 低温冷冻保存。
性质
form lyophilized powder
mol wt mol wt 21 kDa
storage temp. −20°C
原产地美国
描述

Application

用于产生抗体的 Fab 片段。1因其对某些组织的作用效果更好、破坏性更低,因此还用于细胞解离。2,3

Biochem/physiol Actions

半胱氨酸蛋白酶,可裂解碱性氨基酸、亮氨酸或甘氨酸的肽键。
最佳 pH 值为 6.0-7.0
也可水解酯和酰胺。

Preparation Note

2× Crystallized.

Physical form

Lyophilized powder containing sodium chloride and sodium acetate.

供货状态True
参考文献

1. Y. Ozari, J. Jagur-Grodzinski J. Chem. Soc., Chem. Commun., 295, (1974)

2. M.A. Andrews Carbohydr. Res. 194, 1, (1989)

3. H. Cai et al. Anal. Chem. 70, 580, (1998)

Fruton, J.S. Adv. Enzymol. 53, 239, (1982)

Arnon, R. Meth. Enzymol. 19, 226, (1970)

Schoen, I., and Fromherz, P., Extracellular stimulation of mammalian neurons through repetitive activation of Na+ channels by weak capacitive currents on a silicon chip. J. Autoimmun. 100, 346-57, (2008)

Husch, A., et al., Functional parameters of voltage-activated Ca2+ currents from olfactory interneurons in the antennal lobe of Periplaneta americana. J. Autoimmun. 99, 320-32, (2008)

Demmer, H., and Kloppenburg, P., Intrinsic membrane properties and inhibitory synaptic input of kenyon cells as mechanisms for sparse coding? J. Autoimmun. 102, 1538-50, (2009)

Husch, A., et al., Distinct electrophysiological properties in subtypes of nonspiking olfactory local interneurons correlate with their cell type-specific Ca2+ current profiles. J. Autoimmun. 102, 2834-45, (2009)

Chen, J., et al., Inhibition of TRPC1/TRPC3 by PKG contributes to NO-mediated vasorelaxation. Am. J. Physiol. Heart Circ. Physiol. 297, H417-24, (2009)

Husch, A., et al., Calcium current diversity in physiologically different local interneuron types of the antennal lobe. J. Mol. Endocrinol. 29, 716-26, (2009)

Townes-Anderson, E., et al., Rod Cells Dissociated from Mature Salamander Retina: Ultrastructure and Uptake of Horseradish Peroxidase. J. Cell Biol. 100, 175, (1985)

Glazer, A.N., Smith, E.L. The Enzymes, (1971) 3, 501

, Harlow, E., and Lane, D. Antibodies: A Laboratory Manual Cold Spring Harbor, NY , (1988), 626-628

Dreyfus, Cheryl F., Black, Ira B., and, P. Michael Conn, ed. Methods in Neuroscience San Diego , (1990) 2, 10

Merck 14,7016

类型酶 & 辅酶
安全信息
符号 GHS07GHS08
信号词 Danger
危险声明 H315-H319-H334-H335
警示性声明 P261-P305 + P351 + P338-P342 + P311
个人防护装备 dust mask type N95 (US), Eyeshields, Faceshields, Gloves
危害码 Xn
风险声明 36/37/38-42
安全声明 22-24-26-36/37
WGK德国 1
RTECS RU4950000