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中文名称英文名称CAS 编号规格目录价所属品牌订货号
TPCK 处理的胰蛋白酶Trypsin from bovine pancreas,TPCK treated9002-07-750MG452.00SIGMAT8802
TPCK 处理的胰蛋白酶Trypsin from bovine pancreas,TPCK treated9002-07-7100MG795.00SIGMAT8802
TPCK 处理的胰蛋白酶Trypsin from bovine pancreas,TPCK treated9002-07-71G4,600.00SIGMAT8802
品牌SIGMA
产品名称 TPCK 处理的胰蛋白酶 : Trypsin from bovine pancreas,TPCK treated ——【产品编号:SIGMA-T8802】
CAS编号9002-07-7
别名质谱级黄金胰蛋白酶,Trypsin TPCK treated,TPCK胰酶,TPCK胰蛋白酶
级别科学级
纯度&分析TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein
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分子式N/A
分子量N/A
储存条件-20 °C 低温冷冻保存。
性质

sterility    aseptically filled 
form    essentially salt-free, lyophilized powder 
mol wt    mol wt 23.8 kDa 
composition    protein, ≥95% 
solubility    hydrochloric acid: soluble1 mM, clear 
foreign activity    Chymotrypsin ≤0.1 BTEE units/mg protein 
storage temp.    −20°C 

原产地美国
描述

Analysis Note

Protein determined by E1%/280

General description

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

Preparation Note

TPCK treated

Unit Definition

1 BTEE 单位 = 320 ATEE 单位

One BAEE unit will produce a ΔA253 of 0.001 per min at pH 7.6 at 25 °C using BAEE as substrate. Reaction volume = 3.2 ml (1 cm light path).

Application

For trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide. The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsins have also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestions. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps.

Trypsin can be used to release adherent cells from tissue culture plates for passaging. Trypsin has been used in a study to assess the effects of macromolecular crowding on the structural stability of human α-lactalbumin. [1] Trypsin has also been used in a study to investigate BN-PAGE analysis of Trichoderma harzianum secretome. [2]

Biochem/physiol Actions

Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxyl side of the cleavage site. The optimal pH for trypsin activity is 7-9. Trypsin can also act to cleave ester and amide linkages of synthetic derivatives of amino acids. EDTA is added to trypsin solutions as a chelating agent that neutralizes calcium and magnesium ions that obscure the peptide bonds on which trypsin acts. Removing these ions increases the enzymatic activity.

Serine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF, and aprotinin, amongst others, will inhibit Trypsin.

供货状态True
参考文献N/A
类型酶 & 辅酶
安全信息

符号   GHS07GHS08  GHS07, GHS08 
信号词   Danger 
危害声明   H315-H319-H334-H335 
警示性声明   P261-P305 + P351 + P338-P342 + P311 
个人防护装备   dust mask type N95 (US), Eyeshields, Faceshields, Gloves 
危害码 (欧洲)   Xn 
风险声明 (欧洲)   36/37/38-42 
安全声明 (欧洲)   22-24-26-36/37 
RIDADR   NONH for all modes of transport 
WGK德国   1 
RTECS   YN5075000