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中文名称英文名称CAS 编号规格目录价所属品牌订货号
分散酶 IDispase I42613-33-22MG¥1,396.00SIGMAD4818
分散酶 IDispase I42613-33-210MG¥4,880.00SIGMAD4818
分散酶 IDispase I42613-33-225MG¥8,660.00SIGMAD4818
品牌SIGMA
产品名称 分散酶 I : Dispase I ——【产品编号:SIGMA-D4818】
CAS编号42613-33-2
别名Protease from Bacillus polymyxa
级别科学级
纯度&分析protease,≥10 unit/mg solid
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分子式N/A
分子量N/A
储存条件2-8 °C 冷藏保存。
性质
Related Categories Application Index, Biochemicals and Reagents, Cell Biology, Dissociation, Enzymes, Inhibitors, and Substrates,
General Stem Cell Biology, Proteases, Proteases & Protein Sequencing, Stem Cell Biology, Stem Cell Isolation
activity   ≥10 unit/mg solid
storage temp.   2-8°C
原产地美国
描述
Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

Physical form

lyophilized powder containing calcium acetate

Application

Dispase I has been used in a study to assess the effect of amniotic membrane on wound size in the early stages of the healing process. Dispase I has also been used in a study to investigate a dityrosine-based substrate for a protease assay.

Dispase I has been used in lung digestion and processing for flow staining, as well as for CD4 cell isolation in mice. The enzyme has also been used to digest excised wounds and a small amount of surrounding skin for the detection of GFP+ (green fluorescence protein) cells. This study to investigated the effect of differentiation and angiogenesis of bone marrow-derived mesenchymal stem cells on wound healing. It has also been used to remove the epidermis during the isolation of dermal fibroblasts from mice.

Biochem/physiol Actions

Dispase I is a rapid, effective, gentle and neutral protease that can separate intact epidermis from the dermis. It can also separate intact epithelial sheets in culture from the substratum. The enzyme preserves the viability of the epithelial cells while cleaving the basement membrane zone region. It can also be used to prevent clumping in suspension cultures. This protease cleaves fibronectin and type IV collagen, but not laminin, type V collagen, serum albumin, or transferrin.1 It hydrolyzes N-terminal peptide bonds of non-polar amino acid residues. It preferentially attacks denatured and intercellular proteins with exposed hydrophobic amino acid residues. Ca2+, Mg2+, Mn2+, Fe2+, Fe3+ and Al3+ activate the enzyme. EDTA, EGTA, Hg2+ and other heavy metals inhibit the enzyme activity.2 The enzyme contains 1g-atom of zinc per g-mol of purified enzyme. If this zinc component is removed by chelating agents such as EDTA or EGTA, an inactive apoenzyme is obtained. The enzyme is not inhibited by serum.

供货状态True
参考文献

*In the latter application, cells should be added to the gel prior to plating at a recommended density of 3-4 x 104 cells per mL. To dissociate cells from the gel, use protease (dispase) in PBS witho...
George Sitterley
BioFiles 2008, 3.8, 7.
Keywords: Cell culture, Growth factors

类型酶 & 辅酶
安全信息
符号  GHS07GHS08  
信号词  Danger
危险声明  H315-H319-H334-H335
警示性声明  P261-P305 + P351 + P338-P342 + P311
危害码 (欧洲)  Xn
风险声明 (欧洲)  36/37/38-42
安全声明 (欧洲)  22-24-26-36/37
WGK德国  3