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中文名称英文名称CAS 编号规格目录价所属品牌订货号
氨基黑 10BNaphthol Blue Black1064-48-85G¥174.00SIGMAN3393
氨基黑 10BNaphthol Blue Black1064-48-825G¥389.00SIGMAN3393
氨基黑 10BNaphthol Blue Black1064-48-8100G¥972.00SIGMAN3393
品牌SIGMA
产品名称 氨基黑 10B : Naphthol Blue Black ——【产品编号:SIGMA-N3393】
CAS编号1064-48-8
别名苯胺蓝黑,酸性黑1,萘酚蓝黑,酸性黑10B,Acid Black 1,Amido Black 10B,Buffalo Black NBR
级别生化级
纯度&分析BioReagent, suitable for electrophoresis
Tips一种常用的生物染色剂,可用于对聚丙烯酰胺凝胶、琼脂糖凝胶和硝酸纤维素膜上蛋白质的染色。
分子式C22H14N6Na2O9S2
分子量616.49
储存条件常温避光保存。
性质
Related Categories Other Stains for Electrophoresis, Protein Electrophoresis, Protein Stains and Staining Reagents, Proteomics
product line   BioReagent
composition   Dye content, ~85%
total impurities   ≤10% water
suitability   suitable for electrophoresis
原产地美国
描述

Application

Naphthol blue black dye can be used to stain proteins on polyacrylamide gels, agarose gels and nitrocellulose membranes. After electrophoresis, fixing the proteins in the gel is recommended. The gel can then be stained with 0.1% Naphthol Blue Black in 7% (v/v) acetic acid for at least 2 hr and destained with a soluion of 7% (v/v) acetic acid. Detection sensitivity is approx. 20% that of Coomassie Blue R.

供货状态True
参考文献

De Vito, E. and Santome, J., Disc electrophoresis of proteins in the presence of sodium dodecyl sulphate. Experientia 22, 124-125, (1966)

Towbin, H., et al., Electrophoretic transfer of proteins form polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. U. S. A. 76, 4350-4354, (1979)

Wilson, C.M., Staining of proteins on gels: comparisons of dyes and procedures. Meth. Enzymol. 91, 236-247, (1983)

K. Selvam , K. Swaminathan, Decolourization of azo dyes and a dye industry effluent Bioresour. Technol. 88, 115-119, (2003)

B. Mundkur and B.Brauer, Selective Localization of Nucleolar Protein With Amido Black lob J. Histochem. Cytochem. 14(1), (1966)

Beil. 16,IV,622

Corp MSDS 1 (2), 2505:A / FT-IR 2 (3), 4108:A / FT-IR 1 (2), 990:C / IR-Spectra (3), 1464:B / RegBook 1 (2), 2759:O / Sigma FT-IR 1 (2), 181:C / Stains and Dyes Ref, 496 / Structure Index 1, 437:B:8

类型染色剂
安全信息
符号  GHS07  
信号词  Warning
危险声明  H315-H319-H335
警示性声明  P261-P305 + P351 + P338
个人防护装备  dust mask type N95 (US), Eyeshields, Gloves
危害码  Xi
风险声明  36/37/38
安全声明  26
WGK德国  3
RTECS  QJ6196000