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中文名称英文名称CAS 编号规格目录价所属品牌订货号
固绿Fast Green FCF2353-45-95G¥392.00SIGMA-ALDRICHF7258
固绿Fast Green FCF2353-45-925G¥1,564.00SIGMA-ALDRICHF7258
固绿Fast Green FCF2353-45-9100G¥4,876.00SIGMA-ALDRICHF7258
品牌SIGMA-ALDRICH
产品名称 固绿 : Fast Green FCF ——【产品编号:SIGMA-ALDRICH-F7258 】
CAS编号2353-45-9
别名固绿FCF,碱性艳绿,灿烂绿,快绿,Food green 3
级别生物染色剂级
纯度&分析certified by the Biological Stain Commission
Tips一种常用的生物染色剂,可用于IEF、PAGE 和 SDS-PAGE中的蛋白质染色。
分子式C37H34N2O10S3Na2
分子量808.85
储存条件常温避光保存。
性质

Appearance (Color) :Red to Red-Brown
Appearance (Form) :Powder
Solubility (Color) :Blue to VeryDark Blue or Dark Blue-Green,1 mg/mL, H2O
Infrared spectrum :Conforms to Structure
% Dye content :>_ 90 %

原产地美国
描述

Application

Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.

Suitability

Certified for the safranin-fast green FCF stain for plant cells; as a substitute for aniline blue in Gomori′s one-step trichrome procedure.

供货状态True
参考文献

Penny, DP et al, Analysis and testing of biological stains - The Biological Stain Commission Procedures Biotech. Histochem. 77(5&6), 237-275, (2002)

Allen, R.E., et al., Staining protein in isoelectric focusing gels with fast green. Anal. Biochem. 104, 494-498, (1980)

Wilson, C.M., Staining of proteins on gels: comparisons of dyes and procedures. Meth. Enzymol. 91, 236-247, (1983)

Merck 14,3941

Beil. 16,II,513

Aldrich MSDS 1, 945:D / Corp MSDS 1 (1), 1678:A / FT-IR 1 (2), 1033:C / FT-IR 2 (3), 4134:B / IR-Spectra (3), 1485:D / RegBook 1 (2), 2779:I / Sax 6, 1411 / Sigma FT-IR 1 (2), 141:B / Stains and Dyes Ref, 345 / Structure Index 1, 442:B:2

类型染色剂
安全信息
符号  GHS08
信号词  Warning
危险声明  H341
警示性声明  P281
个人防护装备  Eyeshields, Gloves
危害码  Xn
风险声明 (欧洲)  68
安全声明 (欧洲)  36/37
WGK德国  2
RTECS  BQ4425000