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小牛胸腺 DNADeoxyribonucleic acid sodium salt from calf thymus73049-39-550MG¥452.00SIGMAD1501
小牛胸腺 DNADeoxyribonucleic acid sodium salt from calf thymus73049-39-5500MG¥2,424.00SIGMAD1501
小牛胸腺 DNADeoxyribonucleic acid sodium salt from calf thymus73049-39-55G¥14,016.00SIGMAD1501
品牌SIGMA
产品名称 小牛胸腺 DNA : Deoxyribonucleic acid sodium salt from calf thymus ——【产品编号:SIGMA-D1501】
CAS编号73049-39-5
别名DNA钠盐来源于小牛胸腺,ctDNA,胸腺核酸钠盐,脱氧核糖核酸钠盐来源于小牛胸腺
级别科学级
纯度&分析Type I, fibers
Tips一种常见的核酸类衍生物。
分子式N/A
分子量N/A
储存条件2-8 °C 冷藏保存。
性质
APPEARANCE WHITE SOLID
SOLUBILITY CLEAR TO HAZY, COLORLESS TO FAINT YELLOW SOLUTION AT 2 MG/ML IN H20.
SOURCE CALF THYMUS
RATIO A260/A280 > OR = 1.8
A260 UNITS/MG SOLID > OR = 16.0
UNIT DEFINITION ONE UNIT WILL YIELD AN A260 OF 1.0 IN 1.0ML OF 15MM SODIUM CHLORIDE AND 1.5MM SODIUM CITRATE, PH7.0 (1CM LIGHT PATH). ONE MG OF DNA IS EQUIVALENT TO APPROX. 20A260 UNITS.
ICP-ATOMIC EMISSION 6 TO 13% SODIUM
  5 TO 9% PHOSPHORUS
PROTEIN BY LOWRY < OR = 10%
ENZYMATIC SUITABILITY TESTED AND FOUND SUITABLE AS A SUBSTRATE FOR DNASE
原产地美国
描述

Preparation Note

This product is extracted using a method that causes shearing yielding a highly polymerized mixture of double and single stranded DNA . However, double stranded DNA is the predominant form.

This product is prepared from calf thymus tissue of unspecified gender.

Quality

"Highly Polymerized"

Reconstitution

Solutions of DNA have been stored successfully for several months at 4 C, pH 7.5-8, in 10 mM Tris with 1 mM EDTA and without a bacteriostatic agent. At low concentrations (μg/ml) DNA tends to absorb onto the surfaces of plastic tubes. It is not recommended to store DNA in highly alkaline solutions since DNA tends to degrade at alkaline pH greater than 8.0.

This product can be dissolved at 2 mg/ml in water. To reduce further shearing of the DNA, no sonication or stirring should be used. Gentle inversion overnight at 0-4 °C is recommended to completely solubilize the DNA. The presence of 1 mM EDTA is recommended to prevent nucleases from degrading the DNA.

Physical form

fibrous preparation

Application

Calf thymus DNA (CT-DNA) is a natural DNA widely used in studies of DNA binding anticancer agents and DNA binding agents that modulate DNA structure and function. Calf thymus DNA is also used in physicochemical studies of DNA behavior in solution.

Physical properties

λmax 259 nm (100 mM phosphate buffer, pH 7.0).
DNA from calf thymus is 41.9 mole % G-C and 58.1 mole % A-T.1 An OD of 1.0 at 260 nm corresponds to ~ 50 μg of double-stranded DNA.

供货状态True
参考文献

Roginskaya M, Razskazovskiy Y., Selective radiation-induced generation of 2-deoxyribonolactone lesions in DNA mediated by aromatic iodonium derivatives. Radiat. Res. 171, 342-348, (2009)

Tarushi A, Polatoglou E, et al., Interaction of Zn(ii) with quinolone drugs: Structure and biological evaluation. Dalton Trans. 40, 9461-9473, (2011)

Wang MQ, Zhang J, et al., Metal-free cleavage efficiency toward DNA by a novel PNA analog-bridged macrocyclic polyamine. Bioorg. Med. Chem. Lett. 21(19), 5866-5869, (2011)

Gilroy EL, Hicks MR, Smith DJ, Rodger A., Viscosity of aqueous DNA solutions determined using dynamic light scattering. Analyst 136(20), 159-63, (2011)

Ahmadi F, Jamali N, Moradian R, Astinchap B., Binding Studies of Pyriproxyfen to DNA by Multispectroscopic Atomic Force Microscopy and Molecular Modeling Methods. DNA Cell. Biol. 31(2), 259-268, (2011)

Azab HA, Anwar ZM, et al., New Luminescent Bioprobes Eu(lll)-phloroglucinol Derivatives and Their Spectrofluorimetric, Electrochemical Interactions with Nucleotides and DNA. J. Fluoresc. 22(1), 223-238, (2011)

Liu Z, Lv H, Li H, Zhang Y, et al., Interaction Studies of an Anticancer Alkaloid, (+)-(13aS)-Deoxytylophorinine, with Calf Thymus DNA and Four Repeated Double-Helical DNAs. Chemotherapy 57(4), 310-320, (2011)

Camp CL, Sharp BL, Reid HJ, et al., Analysis of mono-phosphate nucleotides as a potential method for quantification of DNA using high performance liquid chromatography-inductively coupled plasma-mass spectrometry. Anal. Bioanal. Chem 402(1), 367-372, (2011)

类型核酸 & 衍生物
安全信息
个人防护装备 Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
安全声明 22-24/25
WGK德国 3