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中文名称英文名称CAS 编号规格目录价所属品牌订货号
TPCK 处理的胰蛋白酶Trypsin from bovine pancreas,TPCK treated9002-07-7100MG¥744.00SIGMAT1426
TPCK 处理的胰蛋白酶Trypsin from bovine pancreas,TPCK treated9002-07-7500MG¥2,170.04SIGMAT1426
TPCK 处理的胰蛋白酶Trypsin from bovine pancreas,TPCK treated9002-07-71G¥3,744.85SIGMAT1426
TPCK 处理的胰蛋白酶Trypsin from bovine pancreas,TPCK treated9002-07-75G¥14,892.54SIGMAT1426
TPCK 处理的胰蛋白酶Trypsin from bovine pancreas,TPCK treated9002-07-7100G¥212,141.24SIGMAT1426
品牌SIGMA
产品名称 TPCK 处理的胰蛋白酶 : Trypsin from bovine pancreas,TPCK treated ——【产品编号:SIGMA-T1426】
CAS编号9002-07-7
别名质谱级黄金胰蛋白酶,Trypsin TPCK treated
级别科学级
纯度&分析≥10,000 BAEE units/mg protein,TPCK Treated, essentially salt-free
Tips此产品经甲苯磺酰苯丙氨酰氯甲酮(TPCK)处理,灭活了残留的胰凝乳蛋白酶活性,可用于消化蛋白、质谱分析、鉴定、蛋白质组学研究等。
分子式N/A
分子量N/A
储存条件-20 °C 低温冷冻保存。
性质
form essentially salt-free, lyophilized powder
mol wt mol wt 23.8 kDa
foreign activity Chymotrypsin ≤0.1 BTEE units/mg protein
storage temp. −20°C
原产地美国
描述

Analysis Note

1 BTEE 单位 = 320 ATEE 单位

Preparation Note

Derived from New Zealand-sourced pancreas

TPCK treated; the treatment with L-1-Tosylamide-2-phenylethyl chloromethyl ketone (TPCK) reduces the chymotrypsin activity which is usually present in trypsin.
Dialyzed

Unit Definition

One BAEE unit will produce a ΔA253 of 0.001 per min at pH 7.6 at 25 °C using BAEE as substrate. Reaction volume = 3.2 ml (1 cm light path).

供货状态True
参考文献

Ceraul, S.M., et al., Functional characterization and novel rickettsiostatic effects of a Kunitz-type serine protease inhibitor from the tick Dermacentor variabilis. Infect. Immun. 76, 5429-35, (2008)

Zhu, S., et al., Activation of cathepsin L by the cathelin-like domain of protegrin-3. Mol. Immunol. 45, 2531-6, (2008)

Williams, J.P., et al., Hot electron capture dissociation distinguishes leucine from isoleucine in a novel hemoglobin variant, Hb Askew, beta54(D5)Val-->Ile. J. Am. Soc. Mass Spectrom. 20, 1707-13, (2009)

Fong, B.Y., and Norris, C.S., Quantification of milk fat globule membrane proteins using selected reaction monitoring mass spectrometry. J. Agric. Food Chem. 57, 6021-8, (2009)

Newman, J., et al., Practical Aspects of the SAMPL Challenge: Providing an Extensive Experimental Data Set for the Modeling Community. J. Biomol. Screen. 14, 1245-50, (2009)

Li, R., et al., Production of piglets after cryopreservation of embryos using a centrifugation-based method for delipation without micromanipulation. Biogerontology 80, 563-71, (2009)

Deleault, N.R., et al., Species-dependent Differences In Cofactor Utilization For Formation Of The Protease-resistant Prion Protein In Vitro. Biochem. Pharmacol. 49, 3928-34, (2010)

Osawa, M., et al., Conversion Of The Metal-specific Activity Of Escherichia Coli Mn-SOD By Site-directed Mutagenesis Of Gly165Thr. Biochim. Biophys. Acta 1804, 1775-1779, (2010)

Genta, F.A., et al., Phenol Oxidases From Rhodnius Prolixus: Temporal And Tissue Expression Pattern And Regulation By Ecdysone. J. Insect Physiol. 56, 1253-9, (2010)

Fuerer, C., et al., A study on the interactions between heparan sulfate proteoglycans and Wnt proteins. Dev. Dyn. 239, 184-90, (2010)

Schoenherr, R.M., et al., Automated Screening Of Monoclonal Antibodies For SISCAPA Assays Using A Magnetic Bead Processor And Liquid Chromatography-selected Reaction Monitoring-mass Spectrometry. J. Dermatol. Sci. 353, 49-61, (2010)

Merck 14,9795

类型酶 & 辅酶
安全信息
符号 GHS07GHS08
信号词 Danger
危险声明 H315-H319-H334-H335
警示性声明 P261-P305 + P351 + P338-P342 + P311
个人防护装备 dust mask type N95 (US), Eyeshields, Faceshields, Gloves
危害码 Xn
风险声明 36/37/38-42
安全声明 22-24-26-36/37
WGK德国 1
RTECS YN5075000