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中文名称英文名称CAS 编号规格目录价所属品牌订货号
外源凝集素(PHA-E)Lectin from Phaseolus vulgaris (red kidney bean)N/A5MG¥1,482.00SIGMAL8629
外源凝集素(PHA-E)Lectin from Phaseolus vulgaris (red kidney bean)N/A25MG¥4,965.00SIGMAL8629
品牌SIGMA
产品名称 外源凝集素(PHA-E) : Lectin from Phaseolus vulgaris (red kidney bean) ——【产品编号:SIGMA-L8629】
CAS编号N/A
别名PHA,菜豆植物血球凝集素,植物血凝素,植物凝集素,植物外源凝集素,phytoh(a)emagglutinin,phytohemagglutinin,plant hemagglutinin
级别科学级
纯度&分析Erythroagglutinin PHA-E
TipsPHA,又名:植物血球凝集素。此产品为E型PHA,来源于菜豆。
分子式N/A
分子量N/A
储存条件2-8 °C 冷藏保存。
性质
form salt-free, lyophilized powder
potency <10 μg per mL agglutination activity ((with erythryocytes))
composition Protein, ≥80% biuret
storage temp. 2-8°C
原产地美国
描述

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of either human erythrocytes (2% in phosphate buffered saline, pH 6.8) or human leukocytes (107 per mL in saline) after 1 hr incubation at 25 °C.

Biochem/physiol Actions

PHA 由两个分子种类构成,一种是红细胞凝集素 (PHA-E),具有低促有丝分裂活性和高红细胞凝集活性,另一种是白细胞凝集素 (PHA-L),具有高促有丝分裂和白细胞凝集活性,但红细胞凝集活性极低。1,2
PHA-E 不具有血型特异性,但凝集作用受某些寡糖抑制。3PHA-P 是在分离和纯化红细胞凝集素和白细胞凝集素前 PHA 的蛋白质形式。4PHA-M 为粘蛋白形式。4由相应的已纯化凝集素制备偶联物。

Preparation Note

Purified by a modification of the method of Leavitt.5

Quality

Tested for leucoagglutination.

供货状态True
参考文献

1. Yachnin, A. and Svenson, R.H. Immunology 22, 871, (1972)

2. Felsted, et al. J. Biol. Chem. 252, 2967, (1977)

3. Kornfeld, R. and Kornfeld, S. J. Biol. Chem. 245, 2536, (1970)

4. Rigas, D.A. and Osgood, E.E. J. Biol. Chem. 212, 607, (1955)

5. Leavitt, R.D., et al. J. Biol. Chem. 252, 2961, (1977)

类型蛋白质
安全信息
个人防护装备 Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK德国 3