中文名称英文名称CAS 编号规格所属品牌订货号
氯化硝基四氮唑蓝(NBT)Nitrotetrazolium Blue chloride298-83-950MGSIGMA-ALDRICHN6639
氯化硝基四氮唑蓝(NBT)Nitrotetrazolium Blue chloride298-83-9250MGSIGMA-ALDRICHN6639
氯化硝基四氮唑蓝(NBT)Nitrotetrazolium Blue chloride298-83-91GSIGMA-ALDRICHN6639
品牌SIGMA-ALDRICH
产品名称 氯化硝基四氮唑蓝(NBT) : Nitrotetrazolium Blue chloride ——【产品编号:SIGMA-ALDRICH-N6639】
CAS编号298-83-9
别名氯化硝基四氮唑兰,氮蓝四唑,四唑氮蓝,p-Nitro-Blue tetrazolium chloride,p-Nitrotetrazolium blue,NBT,Nitro BT
级别分子生物级
纯度&分析powder, for molecular biology
Tips一种常用的生物染色剂,可用于科研、辅料、规模化生产等。
分子式C40H30N10O6 · 2Cl
分子量817.64
储存条件2-8 °C 冷藏保存。
性质
grade   for microscopy
  for molecular biology
form   powder
solubility   H2O: soluble10 mg/mL (A stock solution at 10mg/ml in water is stable for 1-2 weeks in the dark at 0-4°C.)
  2-methoxyethanol: soluble20 mg/mL
  ethanol: soluble5 mg/mL
storage temp.   2-8°C
原产地美国
描述

Application

In combination with 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and an appropriate reaction buffer, Nitroblue tetrazolium forms a substrate for the colorimetric detection of alkaline phosphatase conjugates in a variety of molecular and cell biology techniques.

供货状态True
参考文献

A tetrazolium method for non-specific alkaline phosphatase. McGadey, J. Histochemie 23, 180-184, (1970)

Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots. Leary, J.J., et al. Proc. Natl. Acad. Sci. U. S. A. 80, 4045-4049, (1983)

Visualization of antigenic proteins on Western blots. Knecht, D.A., and Dimond, R.L. Anal. Biochem. 136(1), 180-184, (1984)

Detection of parvovirus B19 in macerated fetal tissue using in situ hybridization. Walters, C., et al. J. Clin. Pathol. 50(9), 749-754, (1997)

Enhanced immunohistochemical detection of autonomic nerve fibers, cytokines and inducible nitric oxide synthase by light and fluorescent microscopy in rat spleen. Meltzer, J.C., et al. J. Histochem. Cytochem. 45(4), 599-610, (1997)

Beil. 26,IV,1789

类型染色剂
安全信息
符号  GHS07  
信号词  Warning
危险声明  H302
个人防护装备  Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
危害码 (欧洲)  Xn
风险声明 (欧洲)  22
WGK德国  3
RTECS  XF8045000