中文名称英文名称CAS 编号规格所属品牌订货号
5-溴-4-氯-3-吲哚葡萄糖苷X-Gluc (X-GlcA)114162-64-010MGSIGMAB6650
5-溴-4-氯-3-吲哚葡萄糖苷X-Gluc (X-GlcA)114162-64-025MGSIGMAB6650
5-溴-4-氯-3-吲哚葡萄糖苷X-Gluc (X-GlcA)114162-64-0100MGSIGMAB6650
品牌SIGMA
产品名称 5-溴-4-氯-3-吲哚葡萄糖苷 : X-Gluc (X-GlcA) ——【产品编号:SIGMA-B6650】
CAS编号114162-64-0
别名5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt,X-GlcA X-glucuronide CHA salt
级别科学级
纯度&分析reagent for selection of recombinant bacterial clones
TipsBSZH品质推荐:SIGMA-ALDRICH 进口高等级试剂,多重遴选,国际标准严苛测试,全球生物化学产品质量标杆,满足用户更高应用要求。 使用SIGMA-ALDRICH品质优异、表现卓越的试剂类产品,将有助于使您的研究项目更早获益。
分子式C14H13BrClNO7 · C6H13N
分子量521.79
储存条件-20 °C 低温冷冻保存。
性质

~grade   Molecular Biology Reagent
  for molecular biology
InChI Key   JXCKZXHCJOVIAV-CYRSAHDMSA-N
sterility   non-sterile
assay   ≥98% (TLC)
form   powder
solubility   DMF: soluble
suitability   suitable for β-galactosidase test
storage temp.   −20°C

原产地美国
描述

~Application
Chromogenic substrate for β-glucuronidase (GUS) gene detection.
Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype.
General description
X-GlcA is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. X-GlcA is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Principle
When X-GlcA is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of X-GlcA turn an intense blue color, allowing for easy differentiation between lac+ and lac- colonies.

供货状态True
参考文献N/A
类型BSZH 生物化学研究
安全信息

~个人防护装备  Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
RIDADR  NONH for all modes of transport
WGK德国  3