中文名称英文名称CAS 编号规格所属品牌订货号
福林酚Folin & Ciocalteu’s phenol reagentN/A50MLSIGMA-ALDRICHF9252
福林酚Folin & Ciocalteu’s phenol reagentN/A100MLSIGMA-ALDRICHF9252
福林酚Folin & Ciocalteu’s phenol reagentN/A500MLSIGMA-ALDRICHF9252
品牌SIGMA-ALDRICH
产品名称 福林酚 : Folin & Ciocalteu’s phenol reagent ——【产品编号:SIGMA-ALDRICH-F9252】
CAS编号N/A
别名Folin-Phenol
级别科学级
纯度&分析suitable for determination of total protein by Lowry method, 2 N
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分子式N/A
分子量N/A
储存条件常温避光保存。
性质

~Appearance (Turbidity) Clear
Appearance (Color) Faint Yellow to Very Dark Yellow and Faint Green-Yellow to Very
Dark Green-Yellow
Appearance (Form) Solution
Concentration 1.9 - 2.1 N
Suitability Suitable

原产地美国
描述

~concentration   2 N
suitability   suitable for determination of total protein by Lowry method

~Other Notes
Folin & Ciocalteu′s phenol reagent does not contain phenol. Rather, the reagent will react with phenols and non-phenolic reducing substances to form chromogens that can be detected spectrophotometrically.
Linkage
This item is also available as part of a kit.

Application
Folin & Ciocalteu′s phenol reagent is most commonly used in the Lowry method for determining protein concentration.[7][6] It has also been used for the quantification of total phenolics.[10] In this method, protein is pretreated with copper(II) in a modified biuret reagent (alkaline copper solution
stabilized with sodium potassium tartrate). Addition of the phenol reagent generates chromogens that give increasing absorbance between 550-750 nm. Normally, absorbance at the peak (750 nm) or shoulder (660 nm) are used to quantitate protein concentrations between 1-100 mg/ml while absorbance at 550 nm is used to quantitate higher protein concentrations.
In the absence of copper, color intensity is determined primarily by the tyrosine and tryptophan content of the protein, and to a lesser extent by cysteine and histidine. Copper(II) has no effect on color formation by tyrosine, tryptophan, or histidine, but reduces color formation due to cysteine.[7][2][8][4][9]

Many modifications of the original assay procedure have been published,[2] including methods for enhancing the color development,[4][3] for determining the content of insoluble proteins,[7][1] and for automating the procedure.[5] Compounds including many buffers, chelating agents, detergents, and cyclic organic compounds can interfere with the Lowry protein assay.[2]
Folin & Ciocalteu′s phenol reagent can also be used as a spray reagent in chromatographic procedures.

供货状态True
参考文献N/A
类型BSZH 生物化学研究
安全信息

~符号  GHS05  GHS05
信号词  Warning
危害声明  H290-H315-H319
警示性声明  P280-P305 + P351 + P338-P337 + P313
危害码 (欧洲)  C,Xi
风险声明 (欧洲)  35-36/38
安全声明 (欧洲)  26-36/37/39-45
RIDADR  UN 3264 8 / PGIII
WGK德国  1