中文名称英文名称CAS 编号规格所属品牌订货号
高峰淀粉酶Taka-Diastase from Aspergillus oryzae9001-19-825GSIGMA86247
高峰淀粉酶Taka-Diastase from Aspergillus oryzae9001-19-8100GSIGMA86247
高峰淀粉酶Taka-Diastase from Aspergillus oryzae9001-19-81KGSIGMA86247
品牌SIGMA
产品名称 高峰淀粉酶 : Taka-Diastase from Aspergillus oryzae ——【产品编号:SIGMA-86247】
CAS编号9001-19-8
别名淀粉糖化酶,他卡淀粉酶糖化素,α-Amylase from Aspergillus oryzae,1,4-α-D-Glucan-glucanohydrolase,Taka-Amylase A
级别科学级
纯度&分析powder, slightly beige, ~100 U/mg
Tips来源于米曲霉菌。
分子式N/A
分子量N/A
储存条件2-8 °C 冷藏保存。
性质
Related Categories 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Carbohydrate Hydrolysis,
Carbohydrate hydrolysis & PTM analysis, Enzyme Class Index, Enzymes, Inhibitors, and Substrates
form powder
color slightly beige
storage temp. 2-8°C
原产地美国
描述

Unit Definition

One unit corresponds to the amount of enzyme which liberates 1 μmol maltose per minute at pH 6.0 and 25 °C (starch according to Zulkowsky, Fluka No. 85642, as substrate).

Application

Amylases from Aspergillus oryzae are used to prevent staling in the baking industry, clarify haze from fruit juices and alcoholic beverages, and produce glucose and maltose syrup products.1

Biochem/physiol Actions

α-amylase catalyzes the hydrolysis of internal α-1,4-O-glycosidic bonds in starches to release α-anomeric sugars.2

α-amylase is classified as family 13 of the glycosyl hydrolases and is present in archaea, bacteria, plants and animals. a-amylase is an essential enzyme in a-glucan metabolism, acting to catalyse the hydrolysis of α-1,4-glucosidic bonds of glycogen, starch and related polysaccharides. Although all α-amylases possess the same catalytic function, they can vary with respect to sequence. In general, they are composed of three domains: a TIM barrel containing the active site residues and chloride ion-binding site (domain A), a long loop region inserted between the third β strand and the α-helix of domain A that contains calcium-binding site(s) (domain B), and a C-terminal β-sheet domain that appears to show some variability in sequence and length between amylases (domain C) [3]. Amylases have at least one conserved calcium-binding site, as calcium is essential for the stability of the enzyme. The chloride-binding functions to activate the enzyme, which acts by a two-step mechanism involving a catalytic nucleophile base (usually an Asp) and a catalytic proton donor (usually a Glu) that are responsible for the formation of the β-linked glycosyl-enzyme intermediate.

供货状态True
参考文献

1. M.J.E.C. Van der Maarel et al, Properties and applications of starch-converting enzymes of the α-amylase family J. Biotechnol. 94, 137-155, (2002)

2. S. Sivaramakrishnan et al, α-amylases from microbial sources- an overview on recent developments Food Technol. 44, 173-184, (2006)

Zimbelman, R.B., et al., Effects Of Encapsulated Niacin On Evaporative Heat Loss And Body Temperature In Moderately Heat-stressed Lactating Holstein Cows. J. Dairy Sci. 93, 2387-94, (2010)

Bernfeld, P. Meth. Enzymol. 1, 149, (1955)

Merck 14,599 / Merck 14,9031

类型酶 & 辅酶
安全信息
符号 GHS08
信号词 Danger
危险声明 H334
警示性声明 P261-P342 + P311
个人防护装备 dust mask type N95 (US), Eyeshields, Faceshields, Gloves
危害码 Xn
风险声明 42
安全声明 22-24-36/37
WGK德国 3
RTECS BU7435000