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中文名称英文名称CAS 编号规格目录价所属品牌订货号
蛋白酶 KProteinase K from Tritirachium album39450-01-6100MG¥1,413.62SIGMAP6556
蛋白酶 KProteinase K from Tritirachium album39450-01-610X100MG¥10,577.31SIGMAP6556
品牌SIGMA
产品名称 蛋白酶 K : Proteinase K from Tritirachium album ——【产品编号:SIGMA-P6556】
CAS编号39450-01-6
别名Endopeptidase K,Actinase K,Pronase K,Protease K,proteinase K
级别科学级
纯度&分析lyophilized powder, ≥30 units/mg protein
Tipsfrom Tritirachium album。
分子式N/A
分子量N/A
储存条件-20 °C 低温冷冻保存。
性质
Quality Level PREMIUM
form lyophilized powder
mol wt mol wt 28.93 kDa
foreign activity Dnase ≤30 Kunitz units/mg solid
  RNase ≤0.003 Kunitz units/mg solid
storage temp. −20°C
原产地美国
描述

Biochem/physiol Actions

Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.

Unit Definition

One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

Application

Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removal endotoxins bound to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.

供货状态True
参考文献

Buschmann, A., et al. Biochem. Biophys. Res. Commun. 253, 693, (1998)

Kristjansson MM, et al. Eur. J. Biochem. 260, 752, (1999)

Sambrook, J., et al. Molecular Cloning: A Laboratory Manual Cold Spring Harbor, NY , (1982)

Sweeney, P.J. and Walker, J.M., Burrell, M.M., Enzymes of molecular biology. Methods Mol. Biol. Towanam NJ , (1993) 16, 306

类型酶 & 辅酶
安全信息
符号 GHS07GHS08
信号词 Danger
危险声明 H315-H319-H334-H335
警示性声明 P261-P305 + P351 + P338-P342 + P311
个人防护装备 dust mask type N95 (US), Eyeshields, Faceshields, Gloves
危害码 Xn
风险声明 36/37/38-42
安全声明 22-24-26-36/37
WGK德国 1