中文名称英文名称CAS 编号规格所属品牌订货号
溴化乙啶Ethidium bromide(EB)1239-45-81GSIGMAE7637
溴化乙啶Ethidium bromide(EB)1239-45-85GSIGMAE7637
溴化乙啶Ethidium bromide(EB)1239-45-825GSIGMAE7637
品牌SIGMA
产品名称 溴化乙啶 : Ethidium bromide(EB) ——【产品编号:SIGMA-E7637 】
CAS编号1239-45-8
别名溴化乙锭,EB,3,8-二氨基-5-乙基-6-苯基菲啶溴化物,EtBr,胡米溴铵
级别生化级
纯度&分析BioReagent, for molecular biology, powder,≥95%
TipsEB是一种高度灵敏的荧光染色剂,用于观察琼脂糖和聚丙烯酰胺凝胶中的DNA。
分子式 C21H20BrN3
分子量394.31
储存条件常温避光保存。
性质
Related Categories Agarose Blends for Electrophoresis, Biochemicals and Reagents, Core Bioreagents, Ethidium Bromide, Fluorescent Probes, Labels, Particles and Stains,
Fluorescent Stains, Molecular Biology, Nucleic Acid Electrophoresis, Nucleic Acid Electrophoresis Stains, Nucleic Acid Gel Stains, Research Essentials
grade   for molecular biology
product line   BioReagent
form   powder
mp   260-262 °C (dec.)(lit.)
suitability   suitable for gel electrophoresis
原产地美国
描述

Biochem/physiol Actions

溴化乙啶可插入双链 DNA 和 RNA 中,并作为移码诱变剂。它还可与吖啶橙结合用于区分存活的、凋亡的和坏死的细胞。

Reconstitution

对于在电泳后进行凝胶染色,应先用水将储存液样品稀释到 0.5μg/ml,再将凝胶孵育 15-30 分钟。通常无需脱色,但是如果必须降低背景色,则可在水中脱色 15 分钟。然后可在紫外灯箱(254nm 波长)中检测 DNA 条带。也可将溴化乙啶加到凝胶和电泳缓冲液(终浓度 0.5μg/ml)中,并在电泳后立即显影。

Application

Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids.1,2,3 It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA.4 Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.5

Frameshift mutagen which intercalates double-stranded DNA and RNA.

供货状态True
参考文献

1. Moore, S.P. , Sutherland, B.M., A densitometric nondestructive microassay for DNA quantitation. Anal. Biochem. 144, 15, (1985)

2. El-Hamalawi, A., The fluorometric determination of nucleic acids in pea seeds by use of ethidium bromide complexes. Anal. Biochem. 67, 384, (1975)

3. Karsten, U. , Wollenberger, A., Improvements in the ethidium bromide method for direct fluorometric estimation of DNA and RNA in cell and tissue homogenates. Anal. Biochem. 77, 464, (1977)

4. Scaria, P.V. , Shafer, R.H., Binding of ethidium bromide to a DNA triple helix. Evidence for intercalation. J. Biol. Chem. 266, 5417, (1991)

5. Elliott, W.H. Biochem. J. 86, 562, (1963)

Severini, A. , Morgan, A.R., An assay for proteinases and their inhibitors based on DNA/ethidium bromide fluorescence. Anal. Biochem. 193, 83, (1991)

Bauer, W. , Vinograd, J., Interaction of closed circular DNA with intercalative dyes. II. The Free energy of superhelix formation in V40 DNA. Mol. Biol. 47, 410, (1970)

Sambrook, J. Molecular Cloning: A Laboratory Manual Cold Spring Harbor, , (1989) 6-44, 6

Gorman, A. Techniques in Apoptosis: A User’s Guide London, UK , (1996), 7

J. N. A. TETTEY, Intracellular localization and metabolism of the phenanthridinium trypanocide, ethidium bromide, by isolated rat hepatocytes Xenobiotica, (1999) 29, 349-360

Merck 14,4731

Beil. 22,V,11,352

Aldrich MSDS 1, 864:C / Corp MSDS 1 (1), 1549:B / FT-IR 1 (2), 873:B / FT-IR 2 (3), 3935:C / IR-Spectra (3), 1399:A / IR-Spectra (2), 1220:B / RegBook 1 (2), 2635:A / Sax 6, 885 / Sigma FT-IR 1 (2), 136:D / Stains and Dyes Ref, 318 / Structure Index 1, 415:E:4

类型染色剂
安全信息
符号  GHS06GHS08
信号词  Danger
危险声明  H302-H330-H341
警示性声明  P260-P281-P284-P310
个人防护装备  Eyeshields, Faceshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges
危害码  T+
风险声明 (欧洲)  22-26-68
安全声明 (欧洲)  28-36/37-45-63
RIDADR  UN 2811 6.1/PG 1
WGK德国  3
RTECS  SF7950000