品牌 |  |
产品名称 |
噻唑蓝(MTT) : Thiazolyl Blue Tetrazolium Bromide ——【产品编号:SIGMA-M5655】
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CAS编号 | 298-93-1 |
别名 | 噻唑兰,四甲基偶氮唑盐,MTT,Methylthiazolyldiphenyl-tetrazolium bromide |
级别 | 细胞培养级 |
纯度&分析 | powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC) |
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分子式 | C18H16BrN5S |
分子量 | 414.32 |
储存条件 | 2-8 °C 冷藏保存。 |
性质 | InChI Key AZKSAVLVSZKNRD-UHFFFAOYSA-M
product line BioReagent
assay ≥97.5% (HPLC)
form powder
mp 195 °C (dec.)(lit.)
solubility H2O: soluble5 mg/mL
suitability suitable for cell culture
suitable for insect cell culture
storage temp. 2-8°C
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原产地 | 美国 |
描述 | Application
Use as a colorimetric metabolic activity indicator in cell viability assays.
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.
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供货状态 | True |
参考文献 | N/A |
类型 | 染色剂 |
安全信息 | 符号 GHS07GHS08 GHS07, GHS08
信号词 Warning
危害声明 H315-H319-H335-H341
警示性声明 P261-P281-P305 + P351 + P338
个人防护装备 Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
危害码 (欧洲) Xn
风险声明 (欧洲) 36/37/38-68
安全声明 (欧洲) 26-36/37
RIDADR NONH for all modes of transport
WGK德国 3
RTECS XF8060000
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