中文名称英文名称CAS 编号规格所属品牌订货号
噻唑蓝(MTT)Thiazolyl Blue Tetrazolium Bromide298-93-1100MGSIGMAM5655
噻唑蓝(MTT)Thiazolyl Blue Tetrazolium Bromide298-93-1250MGSIGMAM5655
噻唑蓝(MTT)Thiazolyl Blue Tetrazolium Bromide298-93-11GSIGMAM5655
噻唑蓝(MTT)Thiazolyl Blue Tetrazolium Bromide298-93-15X1GSIGMAM5655
品牌SIGMA
产品名称 噻唑蓝(MTT) : Thiazolyl Blue Tetrazolium Bromide ——【产品编号:SIGMA-M5655】
CAS编号298-93-1
别名噻唑兰,四甲基偶氮唑盐,MTT,Methylthiazolyldiphenyl-tetrazolium bromide
级别细胞培养级
纯度&分析powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)
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分子式C18H16BrN5S
分子量414.32
储存条件2-8 °C 冷藏保存。
性质

InChI Key   AZKSAVLVSZKNRD-UHFFFAOYSA-M
product line   BioReagent
assay   ≥97.5% (HPLC)
form   powder
mp   195 °C (dec.)(lit.)
solubility   H2O: soluble5 mg/mL
suitability   suitable for cell culture
  suitable for insect cell culture
storage temp.   2-8°C

原产地美国
描述

Application
Use as a colorimetric metabolic activity indicator in cell viability assays.
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.

供货状态True
参考文献N/A
类型染色剂
安全信息

符号  GHS07GHS08  GHS07, GHS08
信号词  Warning
危害声明  H315-H319-H335-H341
警示性声明  P261-P281-P305 + P351 + P338
个人防护装备  Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
危害码 (欧洲)  Xn
风险声明 (欧洲)  36/37/38-68
安全声明 (欧洲)  26-36/37
RIDADR  NONH for all modes of transport
WGK德国  3
RTECS  XF8060000