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中文名称英文名称CAS 编号规格目录价所属品牌订货号
3,3′,5,5′-四甲基联苯胺3,3′,5,5′-Tetramethylbenzidine(TMB)54827-17-7100MG¥246.00ALDRICH860336
3,3′,5,5′-四甲基联苯胺3,3′,5,5′-Tetramethylbenzidine(TMB)54827-17-71G¥420.00ALDRICH860336
3,3′,5,5′-四甲基联苯胺3,3′,5,5′-Tetramethylbenzidine(TMB)54827-17-75G¥2,050.00ALDRICH860336
3,3′,5,5′-四甲基联苯胺3,3′,5,5′-Tetramethylbenzidine(TMB)54827-17-725G¥4,800.00ALDRICH860336
品牌ALDRICH
产品名称 3,3′,5,5′-四甲基联苯胺 : 3,3′,5,5′-Tetramethylbenzidine(TMB) ——【产品编号:ALDRICH-860336】
CAS编号54827-17-7
别名TMB染色剂,BM blue,Sure Blue,TMB,TMB Blotting Plus,MB substrate TMB
级别生物染色剂级
纯度&分析≥99%
Tips一种常用的生物染色剂,可用于科研、辅料、规模化生产等。
分子式 [-C6H2(CH3)2-4-NH2]2
分子量240.34
储存条件常温避光保存。
性质
assay   ≥99%
mp   168-171 °C(lit.)
原产地美国
描述

Application

本品为联苯胺1的非致癌性替代品(Ames 试验阴性),适合作为酶联免疫吸附分析的过氧化物酶底物。底物产生一种可溶性淡蓝色终产物,该产物可通过分光光度法在 370 或 620-650nm 处读取。可用 2M H2SO4 终止 TMB 反应(变成黄色),并在 450nm 处读取数据。一种灵敏的特异性试剂,用于检测血液、2测定血红蛋白、3,4,5测定过氧化物酶。6,7,8

供货状态True
参考文献

1. Holland, V.R., et al. Tetrahedron 30, 3299, (1974)

2. D.D. Garner et al. J. Forensic Sci. 21, 819, (1976)

3. Use of tetramethylbenzidine in plasma hemoglobin assay. J.C. Standefer, D. Vanderjagt Clin. Chem. 23, 749, (1977)

4. Tetramethylbenzidine--a substitute for benzidine in hemoglobin analysis. R.C. Lijana, M.C. Williams J. Lab. Clin. Med. 94, 266, (1979)

5. Quantitative determination of hemoglobin and cytochemical staining for peroxidase using 3,3',5,5'-tetramethylbenzidine dihydrochloride, a safe substitute for benzidine. Liem, H.H. et al. Anal. Biochem. 98, 388, (1979)

6. 3,3',5,5' - Tetramethylbenzidine as an Ames test negative chromogen for horse-radish peroxidase in enzyme-immunoassay. Bos, E.S., et al. J. Immunoassay 2, 187, (1981)

7. Quantitative determination of myeloperoxidase using tetramethylbenzidine as substrate. P.C. Andrews, N.I. Krinsky Anal. Biochem. 127, 346, (1982)

8. Horseradish peroxidase: kinetic studies and optimization of peroxidase activity determination using the substrates H2O2 and 3,3',5,5'-tetramethylbenzidine. H. Gallati, I. Pracht J. Clin. Chem. Clin. Biochem. 23, 453, (1985)

Aldrich MSDS 1, 1679:B / Corp MSDS 1 (2), 3292:A / FT-IR 2 (2), 2090:C / FT-IR 1 (1), 1236:D / FT-NMR 1 (2), 529:B / FT-NMR 1 (1), 1236:D / IR-Spectra (3), 740:D / RegBook 1 (1), 1423:J / Sigma FT-IR 1 (2), 218:D / Stains and Dyes Ref, 693 / Structure Index 1, 222:D:3

类型染色剂
安全信息
符号  GHS07
信号词  Warning
危险声明  H315-H319-H335
警示性声明  P261-P305 + P351 + P338
个人防护装备  dust mask type N95 (US), Eyeshields, Faceshields, Gloves
危害码 (欧洲)  Xi
风险声明 (欧洲)  36/37/38
安全声明 (欧洲)  26-36
WGK德国  3
RTECS  DV2300000