| 品牌 |  |
| 产品名称 |
人胶原蛋白 ： Collagen from human placenta ——【产品编号：SIGMA-C7521】
|
| CAS编号 | 9007-34-5 |
| 别名 | Collagen human，human Collagen，Collagen Type 4 |
| 级别 | 科学级 |
| 纯度&分析 | Bornstein and Traub Type IV（Sigma Type VI）， powder |
| Tips | 4型胶原蛋白，来源于人胎盘，可用于生化分析，细胞培养等研究领域。 |
| 分子式 | N/A |
| 分子量 | N/A |
| 储存条件 | -20 °C 低温冷冻保存。 |
| 性质 |
|
description |
Sigma Type VI |
|
form |
powder |
|
total impurities |
HIV and hepatitis B, none detected |
|
solubility |
0.25% acetic acid: soluble1 mg/mL |
|
storage temp. |
−20°C |
|
| 原产地 | 美国 |
| 描述 |
Analysis Note
Yields 3 major bands following SDS polyacrylamide gel electrophoresis under reducing conditions consistent with basement membrane collagen.
Application
Can be used for the attachment and culturing of epithelial cells, endothelial cells, muscle cells, nerve cells.
General description
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.
Type IV collagen is highly expressed in chorionic villi cytotrophoblast cells. The same cells are also capable of expressing the cleaving enzyme, 70S type IV collagenase.1
Preparation Note
Prepared by a modification of the pepsin extraction method of Glanville, R.W., et al.2
Biochem/physiol Actions
Type IV collagen is a sheet-forming type of collagen found in basement membranes where it serves a critical scaffolding function. In mammals, six proteins make up the type IV collagen family with the distribution of family members being tissue-specific. Structurally, three collagen IV alpha chains assemble to form a flexible heterotrimeric protomer that can self-assemble into network polymers.
Tissue injury in the autoimmune disease Goodpasture syndrome is due to pathogenic autoantibodies targeting the Collagen IV α3 chain . Mutations in COL4A5 are associated with Alport syndrome.
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| 供货状态 | True |
| 参考文献 |
1. Autio-Harmainen, H., et al., Simultaneous expression of 70 kilodalton type IV collagenase and type IV collagen alpha 1 (IV) chain genes by cells of early human placenta and gestational endometrium Lab. Invest., (1992) 67, 191-200
2. Glanville, R.W., et al., Isolation and characterization of a native placental basement-membrane collagen and its component alpha chains. Eur. J. Biochem. 95(2), 383-389, (1979)
Trinh, N.T., et al., EGF and K+ channel activity control normal and cystic fibrosis bronchial epithelia repair. Am. J. Physiol. Heart Circ. Physiol. 295, L866-80, (2008)
Fulcher, M.L., et al., Novel human bronchial epithelial cell lines for cystic fibrosis research. Am. J. Physiol. Heart Circ. Physiol. 296, L82-91, (2009)
Bornstein, P. and Traub, W. The Proteins, (1979) 4, 411-605
|
| 类型 | 蛋白质 |
| 安全信息 |
|
个人防护装备 |
Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|
危害码 |
B |
|
WGK德国 |
1 |
|