中文名称英文名称CAS 编号规格所属品牌订货号
分散酶 IIDispase II42613-33-21GSIGMAD4693
分散酶 IIDispase II42613-33-25GSIGMAD4693
品牌SIGMA
产品名称 分散酶 II : Dispase II ——【产品编号:SIGMA-D4693】
CAS编号42613-33-2
别名Protease from Bacillus polymyxa
级别科学级
纯度&分析protease,≥0.5 units/mg solid
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分子式N/A
分子量N/A
储存条件2-8 °C 冷藏保存。
性质
Related Categories Application Index, Biochemicals and Reagents, Cell Biology, Dissociation, Enzymes, Inhibitors, and Substrates,
General Stem Cell Biology, Proteases, Proteases & Protein Sequencing, Stem Cell Biology, Stem Cell Isolation
activity   ≥0.5 units/mg solid
solubility   10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble
storage temp.   2-8°C
原产地美国
描述
Analysis Note

The enzyme can be initially dissolved in 50 mM Hepes/KOH pH 7.4, 150 mM NaCl at 10 mg/ml. Further dilutions should be made in the cell culture media most appropriate for the cells being dissociated. Dispase is a metalloproteinase and is activated by divalent cations including Ca2+ , Mg2+, Mn2+ and Fe2+.

Preparation Note

A 50 U/mL stock solution may be prepared by dissolving the powder in a buffer containing 10 mM NaAc (pH 7.5) and 5 mM CaAc. It should be stored at 4 °C.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

Physical form

lyophilized powder containing calcium acetate and milk sugar

Application

The enzyme from Sigma has been used in developing a protocol for ex vivo culture of mouse embryonic mammary buds.1 It has been used in the treatment of rat heart pieces during the isolation of mitochondria from rat heart.2 It has also been used for the isolation of dental pulp stem cells (DPSCs) by enzymatic hydrolysis. These cells have been compared with DPSCs isolated by explant method to analyse their stem cell and differentiation properties.3

Biochem/physiol Actions

Dispase II is a neutral protease that hydrolyzes the N-terminal peptide bonds of non-polar amino acid residues. It may be used for separating many tissues and cells grown in vitro. The enzyme is very gentle and does not damage cell membranes. It can also be used to prevent clumping in suspension cultures. This protease cleaves fibronectin and type IV collagen, but not laminin, type V collagen, serum albumin, or transferrin.4 Dispase II is specific for the cleavage of Leucine-Phenylalanine bonds. Ca2+, Mg2+, Mn2+, Fe2+, Fe3+ and Al3+ activate the enzyme. EDTA, EGTA, Hg2+ and other heavy metals inhibit the enzyme activity.5 The enzyme contains 1g-atom of zinc per g-mol of purified enzyme. If this zinc component is removed by chelating agents such as EDTA or EGTA, an inactive apoenzyme is obtained. The enzyme is not inhibited by serum.

供货状态True
参考文献N/A
类型酶 & 辅酶
安全信息
符号  GHS07GHS08  
信号词  Danger
危险声明  H315-H319-H334-H335
警示性声明  P261-P305 + P351 + P338-P342 + P311
危害码 (欧洲)  Xn
风险声明 (欧洲)  36/37/38-42
安全声明 (欧洲)  22-24-26-36/37
WGK德国  3