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中文名称英文名称CAS 编号规格目录价所属品牌订货号
重组人胰岛素Insulin, Recombinant Human11061-68-025MG¥570.00SIGMA91077C
重组人胰岛素Insulin, Recombinant Human11061-68-0100MG¥1,412.88SIGMA91077C
品牌SIGMA
产品名称 重组人胰岛素 : Insulin, Recombinant Human ——【产品编号:SIGMA-91077C】
CAS编号11061-68-0
别名Recombinant Human
级别科学级
纯度&分析dry powder, for research or for further manufacturing use
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分子式C257H383N65O77S6
分子量5807.57
储存条件-20 °C 低温冷冻保存。
性质

胰岛素(insulin)是胰腺中胰岛β细胞分泌的一种双链形式(α, β)的多肽激素,由51个氨基酸组成,其中α链包含21个氨基酸,β链包含30个氨基酸。两条链间通过二对二硫键连接,而α链自身还含有一对二硫键。胰岛素的分泌过程是:前胰岛素原的氨基末端携带有一段23-30氨基酸的信号肽,该信号肽可将前胰岛素原转移至内质网,然后立即被切断,释放出胰岛素原。胰岛素原由胰岛素β链、C-肽和α链组成,释放出的胰岛素原随即被转至高尔基体,在此被包装并转化成胰岛素,与C-肽一同释放至血液。

胰岛素是目前已知的机体内唯一能降低血糖的蛋白激素,不仅负责调控细胞对葡萄糖、氨基酸和脂肪酸的摄入,利用和储存,而且抑制糖原,蛋白质和脂肪的降解发生。不同物种胰岛素功能大体相同,只是氨基酸组成上的差异。

本品为基因重组技术制备的全长序列的人胰岛素,分子量大约为,结构与天然来源的人胰岛素相同,常用作细胞培养的辅助成分,常见使用浓度为1-10μg/ml。

~Quality Level   GMP
form   dry powder
potency   ≥27.5 units per mg
storage temp.   −20°C

原产地美国
描述

Biochem/physiol Actions
Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat.


Application
Serum-free medium supplements such as insulin are essential for long-term growth of commonly used mammalian cell lines. When insulin is absent from media, cell may exhibit disturbances in morphology and growth rate.
• Recombinant Human Insulin has been used in the media for adipogenic induction of mesenchymal stem cells.[1]
• It has been used as a supplement in the cell culture media of airway epithelial cells.[2]
• It has been used in the culture media for differentiation of cells, GLUT4 (glucose transporter type 4) translocation assay and to study interaction of insulin with insulin receptor via surface plasmon resonance.[3]


General description
Insulin, recombinant human (rHu) is identical in function and structure to the native human sequence. A hormone consisting of two polypeptide chains, rHu Insulin′s A-chain (21 amino acids) and B-chain (30 amino acids) are covalently linked by disulfide bonds between cysteine residues. This genetically engineered human insulin is a full sequence molecule producted by recombinant DNA technology in baker′s yeast.

供货状态True
参考文献N/A
类型BSZH 有机化学试剂
安全信息

个人防护装备  Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
RIDADR  NONH for all modes of transport
WGK德国  nwg
RTECS  NM8900250