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中文名称英文名称CAS 编号规格目录价所属品牌订货号
谷胱甘肽-琼脂糖凝胶 4BGlutathione Sepharose 4BN/A10ML¥3,213.00BSZHGS4B336
谷胱甘肽-琼脂糖凝胶 4BGlutathione Sepharose 4BN/A100ML¥20,930.00BSZHGS4B336
谷胱甘肽-琼脂糖凝胶 4BGlutathione Sepharose 4BN/A1L¥129,700.00BSZHGS4B336
品牌BSZH
产品名称 谷胱甘肽-琼脂糖凝胶 4B : Glutathione Sepharose 4B ——【产品编号:BSZH-GS4B336】
CAS编号N/A
别名谷胱甘肽琼脂糖凝胶4B,Glutathione Sepharose™ 4B
级别定向纯化级
纯度&分析Average particle size:90 µm
Tips此产品适用于科学研究、药用辅料、规模化生产等。
分子式N/A
分子量N/A
储存条件2-8 °C 冷藏保存。
性质

Glutathione Sepharose™ 4B Media/GSTrap 4B Columns - Easy Purification

Fast and simple purification of GST*-tagged proteins.

GSTrap™ 4B 1-ml and 5-ml columns are prepacked with Glutathione Sepharose™ 4B.

GSTrap™ 4B are designed to be used with a syringe, pump, or chromatography system, such as ÄKTAdesign.

原产地美国
描述

GSTrap 4B 1-ml and 5-ml columns are prepacked with Glutathione Sepharose 4B for fast purification of GST-tagged proteins and scale-up.

 

GSTrap™ 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function.

 

Columns: Capture GSTrap 4B 1 ml
Polishing HiLoad 16/60 Superdex 200 pg
Sample: Cell extract from E. coli expressing GST-hippocalcin (Mr 45 000)
after lysis with CelLytic Express
AC binding/wash buffer: 10 mM sodium phosphate, 140 mM, pH 7.4
AC elution buffer: 50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0
GF buffer: 50 mM phosphate buffer, 150 mM NaCl, pH 7.2
System: ÄKTAprime plus


1 Low molecular weight markers
2 Start material (diluted 1:20)
3 Eluted pool GSTrap 4B
4 Eluted pool GSTrap 4B (diluted 1:50)
5 Peak 1, HiLoad 16/60 Superdex 200 pg
6 Peak 2, HiLoad 16/60 Superdex 200 pg

TECHNICAL SPECIFICATIONS
Ligand Glutathione
Ligand coupling method Epoxy activation
Dynamic binding capacity* > 5 mg horse liver GST/ml medium
Matrix Agarose, 4%
Average particle size 90 μm
pH stability 4-13
Storage 20% ethanol
Storage temperature 4°C to 30°C
HiTrap prepacked columns
Column volume 1 ml and 5 ml
Column dimensions 0.7 × 2.5 cm (1 ml); 1.6 × 2.5 cm (5 ml)
Recommended flow rate 0.2-1 ml/min (1 ml); 0.5-5 ml/min (5 ml)
Max. pressure 3 bar (0.3 MPa)
* Binding of GST to glutathione is flow-rate dependent and lower flow rates often iincrease the binding capacity. This is an important consideration, especially during sample loading and elution.
H2O at 25°C.
供货状态True
参考文献N/A
类型GE Healthcare® 系列产品
安全信息
个人防护装备 Eyeshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter
风险声明 10
安全声明 7-16
RIDADR UN 1170 3/PG 3
WGK德国 3