| 描述 |
Application
β-glucosidase is also used in the synthesis of glucosides and fucosides with various potential applications in pharmaceutical, cosmetic and detergent industries1, hydrolytic removal of aglycone moiety from flavonoid and isoflavonoid glycosides2, flavor enhancement of fruit juices and wine3, and biosynthesis of oligosaccharides4.
Biochem/physiol Actions
βglucosidase is involved in the hydrolysis of β-glycosidic bonds connecting carbohydrate residues in β-D-glycosides. They convert cellobiose and cellooligosaccharides produced by the endo and exoglucanases to glucose.5
Quality
Crude.
Unit Definition
One unit will liberate 1.0 μmole of glucose from salicin per min at pH 5.0 at 37 °C.
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| 参考文献 |
1. A Ducret et al., Comparison between various commercial sources of almond β-glucosidase for the production of alkyl glucosides J. Mol. Catal., B Enzym. 38, 91-94, (2006)
2. R. Briante et al., Hydrolysis of oleuropein by recombinant β-glucosidase from hypothermophillic aracheon Sulfolobus solfataricus immobilized on chitosan matrix J. Biotechnol. 77, 275-286, (2000)
3. Y. Guegen et al., Enhancement of aromatic quality of Muscat wine by the use of immobilized β-glucosidase J. Biotechnol. 55, 151-156, (1997)
4. M.E. Bruins et al., Oligosaccharide synthesis by the hyperthermostable βglucosidase from Pyrococcus furiosus: kinetics and modeling Enzyme Microb. Technol. 33, 3-11, (2003)
5. S. Ferreira et al., Response surface optimization of enzymatic hydrolysis of Cistus ladanifer and Cytisus striatus for bio-ethanol production Biochem. Eng. J. 45, 192-200, (2009)
Henzler, K., et al., Enzymatic activity of immobilized enzyme determined by isothermal titration calorimetry. Anal. Biochem. 378, 184-9, (2008)
Bloem, A., et al., Hydrolysis of glycosidically bound flavour compounds from oak wood by Oenococcus oeni. Food Microbiol. 25, 99-104, (2008)
Matsuura, K., et al., Cuckoo fungus mimics termite eggs by producing the cellulose-digesting enzyme beta-glucosidase. Curr. Biol. 19, 30-6, (2009)
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