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中文名称英文名称CAS 编号规格目录价所属品牌订货号
溶细胞酶Lyticase from Arthrobacter luteus37340-57-110KU¥400.00SIGMAL2524
溶细胞酶Lyticase from Arthrobacter luteus37340-57-125KU¥830.00SIGMAL2524
溶细胞酶Lyticase from Arthrobacter luteus37340-57-150KU¥1,560.00SIGMAL2524
溶细胞酶Lyticase from Arthrobacter luteus37340-57-1200KU¥5,900.00SIGMAL2524
品牌SIGMA
产品名称 溶细胞酶 : Lyticase from Arthrobacter luteus ——【产品编号:SIGMA-L2524】
CAS编号37340-57-1
别名溶壁酶
级别科学级
纯度&分析lyophilized powder, ≥2,000 units/mg protein, Protein ≥20 % by biuret
Tips来源于藤黄节杆菌(from Arthrobacter luteus)。
分子式N/A
分子量N/A
储存条件-20 °C 低温冷冻保存。
性质
Quality Level PREMIUM
form lyophilized powder
composition Protein, ≥20% biuret
storage temp. −20°C
原产地美国
描述

Biochem/physiol Actions

Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.

Unit Definition

One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.

Physical form

Partially purified, lyophilized powder containing potassium phosphate buffer salts and stabilizers.

Application

Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase, chitinase, zymolase, and gluculase to induce partial spheroplast formation; spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation.
Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species.

供货状态True
参考文献

Prieto, J., et al., Generation and analysis of mesophilic variants of the thermostable archaeal I-DmoI homing endonuclease. J. Bacteriol. 283, 4364-74, (2008)

Murray, S., and Kiseleva, E., A protocol for isolation and visualization of yeast nuclei by scanning electron microscopy. Methods Cell Biol. 88, 367-87, (2008)

Zenklusen, D., et al., Single-RNA counting reveals alternative modes of gene expression in yeast. Nat. Struct. Mol. Biol. 15, 1263-71, (2008)

Jazwinski, S.M. Meth. Enzymol. 182, 154-174, (1990)

van Burik, J.A., et al. Med. Mycol 36, 299-303, (1998)

Bir, N., et al. Prep. Biochem. 25, 171-181, (1995)

Phalip, V., et al. Biotechnol. Lett. 26, 409-413 , (2004)

类型酶 & 辅酶
安全信息
符号 GHS08
信号词 Danger
危险声明 H334
警示性声明 P261-P342 + P311
个人防护装备 Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
危害码 Xn
风险声明 42
安全声明 22
WGK德国 3