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中文名称英文名称CAS 编号规格目录价所属品牌订货号
噻唑蓝(MTT)Thiazolyl Blue Tetrazolium Bromide298-93-1250MG¥262.00SIGMAM2128
噻唑蓝(MTT)Thiazolyl Blue Tetrazolium Bromide298-93-11G¥450.00SIGMAM2128
噻唑蓝(MTT)Thiazolyl Blue Tetrazolium Bromide298-93-15G¥1,360.00SIGMAM2128
噻唑蓝(MTT)Thiazolyl Blue Tetrazolium Bromide298-93-110G¥2,384.00SIGMAM2128
噻唑蓝(MTT)Thiazolyl Blue Tetrazolium Bromide298-93-125G¥3,900.00SIGMAM2128
品牌SIGMA
产品名称 噻唑蓝(MTT) : Thiazolyl Blue Tetrazolium Bromide ——【产品编号:SIGMA-M2128】
CAS编号298-93-1
别名噻唑兰,四甲基偶氮唑盐,MTT,Methylthiazolyldiphenyl-tetrazolium bromide
级别生物染色剂级
纯度&分析98.0%,HPLC
Tips一种常用的生物染色剂,可用于科研、辅料、规模化生产等。
分子式 C18H16BrN5S
分子量414.32
储存条件2-8 °C 冷藏保存。
性质
assay   98%
mp   195 °C (dec.)(lit.)
storage temp.   2-8°C
原产地美国
描述

Application

Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation.1,2,3,4,5 MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent6 and for the detection of NAD.7 ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used.8 MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.9

供货状态True
参考文献

1. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Mosman J. Immunol. Methods 65, 55, (1983)

2. Tumor suppression by RNA from the 3' untranslated region of α-tropomyosin. Rastinejad., F., et al. Cell 75, 1107-17, (1993)

3. Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. Denizot, F. and Lang, R. J. Immunol. Methods 89, 271, (1986)

4. Evaluation of a tetrazolium-based, semi-automated colorimetric assay: assessment of chemosensitivity testing. Carmicheal, J., et al. Cancer Res. 47, 936-942, (1987)

5. Rapid, modified Kirby-Bauer susceptibility test with single, high-concentration antimicrobial disks. Boyle, V.J., et al. Antimicrob. Agents Chemother. 3, 418-24, (1973)

6. Subtantivity and other factors responsible for formazan patterns in dehydrogenase histochemistry. Pearse, A.G.E., and Hess, R. Experientia 17, 136, (1961)

7. An improved cycling assay for nicotinamide adenine dinucleotide. Bernofsky, C. and Swan, M. Anal. Biochem. 53, 452, (1973)

8. Cytochemical localization of pyridine nucleotide-linked dehydrogenases. Hess, R., et al. Nature 181, 1531-2, (1958)

9. Intracellular localisation of dehydrogenase systems using monotetrazolium salts and metal chelation of their formazans. Pearse, A.G. J. Histochem. Cytochem. 5, 515-27, (1957)

Beil. 27,IV,6045

Corp MSDS 1 (2), 2479:B / FT-IR 2 (3), 3536:A / FT-IR 1 (2), 633:B / FT-NMR 1 (3), 93:B / IR-Spectra (2), 1068:B / IR-Spectra (3), 1227:G / NMR-Reference 2 (2), 501:D / RegBook 1 (2), 2371:L / Sigma FT-IR 1 (2), 171:C / Stains and Dyes Ref, 488 / Structure Index 1, 376:D:5

类型染色剂
安全信息
符号  GHS07GHS08
信号词  Warning
危险声明  H315-H319-H335-H341
警示性声明  P261-P281-P305 + P351 + P338
个人防护装备  Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
危害码 (欧洲)  Xn
风险声明 (欧洲)  36/37/38-68
安全声明 (欧洲)  26-36/37
WGK德国  3
RTECS  XF8060000