中文名称英文名称CAS 编号规格所属品牌订货号
蛋白酶 EProtease from Streptomyces griseus9036-06-0100MGSIGMAP6911
蛋白酶 EProtease from Streptomyces griseus9036-06-01GSIGMAP6911
蛋白酶 EProtease from Streptomyces griseus9036-06-05GSIGMAP6911
品牌SIGMA
产品名称 蛋白酶 E : Protease from Streptomyces griseus ——【产品编号:SIGMA-P6911】
CAS编号9036-06-0
别名链霉蛋白酶E,Actinase E,Pronase E,Protease E,proteinase E
级别科学级
纯度&分析BioReagent, for molecular biology, DNase, RNase, and nickase, none detected (No RNase.)
Tips来源于链球菌属(from Streptomyces griseus)。
分子式N/A
分子量N/A
储存条件-20 °C 低温冷冻保存。
性质
grade for molecular biology
product line BioReagent
activity ≥4 units/mg solid
mol wt monomer mol wt ~20 kDa
foreign activity DNase, RNase, and nickase, none detected (No RNase.)
storage temp. −20°C
原产地美国
描述

Application

Protease is typically used in nucleic acid isolation procedures in incubations of 0.5-3.0 hours supplemented with 0.2% sodium dodecyl sulfate and 10 mM EDTA.

Preparation Note

Collected from culture broth of S. griseus and purified by successive column procedures.

Specificity

A mixture of at least three proteolytic activities including an extracellular serine protease. In general, serine proteases display a wide range of substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule. This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

Physical properties

Completely inactivated by heating above 80 °C for 15-20 minutes.

供货状态True
参考文献

Yoshida, N., et al., Purification and characterization of an acidic amino acid specific endopeptidase of Streptomyces griseus obtained from a commercial preparation (Pronase). J. Biochem. Tokyo 104, 451-456, (1988)

Yum, D.Y., et al., Purification and characterization of alkaline serine protease from an alkalophilic Streptomyces sp. Biosci. Biotechnol. Biochem. 58, 470-474, (1994)

类型酶 & 辅酶
安全信息
符号 GHS07GHS08
信号词 Danger
危险声明 H315-H319-H334-H335
警示性声明 P261-P305 + P351 + P338-P342 + P311
个人防护装备 Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
安全声明 22-24/25
WGK德国 2
RTECS UK9595000