Analysis Note

据报道，凝集活性以 μg/ml 表达，并且在 Ca²⁺ 和 Mn²⁺ 浓度为 1mg/ml、pH 6.8 的磷酸缓冲盐溶液中进行连续稀释测定。该活性是在 25°C 下孵育 1 小时的 2% 人红细胞悬浮液可以凝集的最低浓度。

Biochem/physiol Actions

刀豆素 A 无血型特异性，但对末端 α-D-甘露糖基和 α-D-葡糖基残基具有亲和力。需要 Ca²⁺ 和 Mn²⁺ 离子存在才能显示活性。刀豆素 A 在 pH 6.5 或更低的条件下解离为二聚体。pH 在 5.8 至 7.0 之间时，刀豆素 A 以四聚体形式存在；pH 高于 7.0 时，形成更高的聚合体。根据聚合程度不同，刀豆素 A 可能显示出具有促有丝分裂的活性。琥珀酰化可以生成一种能够在 pH 高于 5.6 时仍保持二聚体形式的活性二聚体。

Preparation Note

Produced from whole Jack beans (J0250) using acetic acid by the method of Olson and Liener.¹

Quality

Highly purified.

1. Olson, M.O.J. and Liener, I.E. Biochemistry 6, 105, (1967)

Ku, H.Y., et al., Organization Of F-actin Via Concerted Regulation Of Kette By PTP61F And DAbl. Mol. Cell. Biol. 29, 3623-32, (2009)

Barnett, J.L., et al., The Effects Of A Perch, Dust Bath, And Nest Box, Either Alone Or In Combination As Used In Furnished Cages, On The Welfare Of Laying Hens. Poult. Sci. 88, 456-70, (2009)

Han, Y., Rutin Has Therapeutic Effect On Septic Arthritis Caused By Candida Albicans. Int. Immunopharmacol. 9, 207-11, (2009)

Drews-Elger, K., et al., The Transcription Factor NFAT5 Is Required For Cyclin Expression And Cell Cycle Progression In Cells Exposed To Hypertonic Stress. PLoS ONE 4, e5245, (2009)

Avril, M., et al., Optimizing Expression Of The Pregnancy Malaria Vaccine Candidate, VAR2CSA In Pichia Pastoris. Malaria Journal 8, 143, (2009)

Varthaman, A., et al., Control Of T Cell Reactivation By Regulatory Qa-1-restricted CD8+ T Cells. J. Immunol. 184, 6585-91, (2010)

Seo, M.J., et al., The Therapeutic Effect Of DA-6034 On Ocular Inflammation Via Suppression Of MMP-9 And Inflammatory Cytokines And Activation Of The MAPK Signaling Pathway In An Experimental Dry Eye Model. Curr. Eye Res. 35, 165-75, (2010)

Reeke, G.N., et al. Ann. N.Y. Acad. Sci. 234, 369, (1974)

Kalb, A.J. and Lustig, A. Biochim. Biophys. Acta 168, 366, (1968)

Gunther, G.R., et al. Proc. Natl. Acad. Sci. U. S. A. 70, 1012, (1973)

, Bittiges, H. and Schnebli, H.P, ed. Concanavalin A as a Tool London , (1976)

Merck 14,2495