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中文名称英文名称CAS 编号规格目录价所属品牌订货号
双(2-羟乙基)氨基-三(羟甲基)甲烷BIS-TRIS6976-37-025G¥395.00SIGMAB4429
双(2-羟乙基)氨基-三(羟甲基)甲烷BIS-TRIS6976-37-0100G¥1,162.00SIGMAB4429
双(2-羟乙基)氨基-三(羟甲基)甲烷BIS-TRIS6976-37-0500G¥5,554.00SIGMAB4429
双(2-羟乙基)氨基-三(羟甲基)甲烷BIS-TRIS6976-37-01KG¥9,564.00SIGMAB4429
双(2-羟乙基)氨基-三(羟甲基)甲烷BIS-TRIS6976-37-05KG¥36,910.00SIGMAB4429
品牌SIGMA
产品名称 双(2-羟乙基)氨基-三(羟甲基)甲烷 : BIS-TRIS ——【产品编号:SIGMA-B4429】
CAS编号6976-37-0
别名2-二(2-羟乙基)氨基-2-羟甲基-1,3-丙二醇,2-双(羟基甲基)-2,2′,2″-次氮基三乙醇,二(2-羟乙基)亚氨基三(羟甲基)甲烷,2,2-双二羟甲基苯胺,2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol,2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol,Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane
级别细胞培养级
纯度&分析≥98% (titration),BioReagent, cell culture tested, suitable for insect cell culture
Tips生化制品,可用于细胞培养,可用于昆虫细胞培养。
分子式C8H19NO5
分子量209.24
储存条件常温避光保存。
性质
Quality Level ELITE
product line BioReagent
assay ≥98% (titration)
total impurities endotoxin and total aerobic microbial count, tested
useful pH range 5.8 - 7.2
pKa (25 °C) 6.5
cation traces heavy metals (as Pb): ≤5 ppm
suitability cell culture tested
  suitable for insect cell culture
原产地美国
描述

Appearance (Color) :White
Appearance (Form) :Crystalline Powder
Solubility (Color) :Colorless to Very Faint Yellow
Solubility (Turbidity),500 mg/ml in H2O :Clear
Water (by Karl Fischer) :1 %
Heavy Metals :<= 5 ppm ,Pb <= 5 ppm
Infrared spectrum :Conforms to Structure
Titration by H2SO4 (anhydrous) :98.0 %
DNAse, Exonuclease Detection :None Detected
NICKase,Endonuclease Detection :None Detected
RNAse Detection :None Detected
Protease Detection :None Detected
Endotoxin Level :1 EU/mg

供货状态True
参考文献

1. Good, N. E., et al, Hydrogen ion buffers for biological research. Biochemistry, 5(2), 467-477 (1966).
2. Molecular Cloning: A Laboratory Manual, 3rd ed., Sambrook, J. and Russell, D. W., CSHL Press (Cold Spring Harbor, NY: 2001), pp. 7.26-7.30.
3. Thevis, M., et al., In-gel derivatization of proteins for cysteine-specific cleavages and their analysis by mass spectrometry. J. Proteome Res., 2(2), 163-172 (2003).
4. Liu, Q., et al., pK-matched running buffers for gel electrophoresis. Anal. Biochem., 270(1), 112-122 (1999).
5. Wiltfang, J., et al., A new multiphasic buffer system for sodium dodecyl sulfate-polyacrylamide gel electrophoresis of proteins and peptides with molecular masses 100,000-1000, and their
detection with picomolar sensitivity. Electrophoresis, 12(5), 352-366 (1991).
6. Strating, H., and Clarke, A. J., Differentiation of bacterial autolysins by zymogram analysis. Anal. Biochem., 291(1), 149-154 (2001).

类型缓冲介质
安全信息
符号 GHS07
信号词 Warning
危险声明 H315-H319-H335
警示性声明 P261-P305 + P351 + P338
个人防护装备 dust mask type N95 (US), Eyeshields, Gloves
危害码 Xi
风险声明 36/37/38
安全声明 26-36
WGK德国 3