中文名称英文名称CAS 编号规格所属品牌订货号
L-谷氨酸脱氢酶(II型)L-Glutamic Dehydrogenase from bovine liver9029-12-320MGSIGMAG2626
L-谷氨酸脱氢酶(II型)L-Glutamic Dehydrogenase from bovine liver9029-12-350MGSIGMAG2626
L-谷氨酸脱氢酶(II型)L-Glutamic Dehydrogenase from bovine liver9029-12-3100MGSIGMAG2626
L-谷氨酸脱氢酶(II型)L-Glutamic Dehydrogenase from bovine liver9029-12-3500MGSIGMAG2626
品牌SIGMA
产品名称 L-谷氨酸脱氢酶(II型) : L-Glutamic Dehydrogenase from bovine liver ——【产品编号:SIGMA-G2626】
CAS编号9029-12-3
别名L-GLDH,L-Glutamate:NAD[P]+, Oxidoreductase (deaminating) ,Glutamate Dehydrogenase from bovine liver
级别科学级
纯度&分析Type II, aqueous glycerol solution, ≥35 units/mg protein
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分子式N/A
分子量N/A
储存条件2-8 °C 冷藏保存。
性质
type   Type II
form   aqueous glycerol solution
shipped in   wet ice
storage temp.   2-8°C
原产地美国
描述
Analysis Note

Protein determined by biuret.

Unit Definition

One unit will reduce 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.3 at 25 °C, in the presence of ammonium ions.

Physical form

Solution in 50% glycerol, pH 7.3

Application

L-glutamic dehydrogenase was used to catalyze the conversion of isocitrate into α-ketoglutarate and carbon dioxide.1

Biochem/physiol Actions

Mammalian forms of this enzyme, including this bovine form, can use either NADP(H) or NAD(H) as coenzymes. L-glutamic dehydrogenase plays a unique role in mammalian metabolism. The reverse reaction catalyzed by this enzyme is the only pathway by which ammonia can become bound to the α-carbon atom of an α-carboxylic acid and thus, is the only source of de novo amino acid synthesis in mammalian species.

The bovine enzyme is characterized by three sets of properties:
• It has a reversible concentration-dependent association, producing higher molecular weight forms.
• Forms tight enzyme-reduced coenzyme-substrate ternary complexes whose rates of dissociation modulate the steady-state reaction rates.
• Exhibits a wide variety of effects from the binding of any of a number of nucleotide modifiers.

L-glutamic dehydrogenase catalyzes the conversion of glutamate to α-ketoglutarate2,3.

供货状态True
参考文献

1. Glutamine supports pancreatic cancer growth through a KRAS-regulated metabolic pathway. Son J, Lyssiotis CA, Ying H, et al. Nature 496(7443), 101-5, (2013)


2. Glutamic dehydrogenase. STRECKER HJ Arch. Biochem. Biophys. 46(1), 128-40, (1953)


3. Regulation of bovine glutamate dehydrogenase. The effects of pH and ADP J. Bailey et al. J. Biol. Chem. 257, 5579-5583, (1982)


4.T he kinetic mechanism of ox liver glutamate dehydrogenase in the presence of the allosteric effector ADP D.P. Hornby et al. Biochem. J. 223, 161-168, (1984)


5. Mitochondrial targeting adaptation of the hominoid-specific glutamate dehydrogenase driven by positive Darwinian selection. Rosso L, Marques AC, Reichert AS, et al. PLoS Genet. 4(8), 1000150, (2008)


6. Spectrum of glutamate dehydrogenase mutations in Japanese patients with congenital hyperinsulinism and hyperammonemia syndrome. Aso K, Okano Y, Takeda T, et al. Osaka City Med. J. 57(1), 1-9, (2011)

类型酶 & 辅酶
安全信息
Personal Protective Equipment:Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK Germany: 3