| 品牌 |  |
| 产品名称 |
L-谷氨酸脱氢酶（II型） ： L-Glutamic Dehydrogenase from bovine liver ——【产品编号：SIGMA-G2626】
|
| CAS编号 | 9029-12-3 |
| 别名 | L-GLDH，L-Glutamate:NAD[P]+， Oxidoreductase (deaminating) ，Glutamate Dehydrogenase from bovine liver |
| 级别 | 科学级 |
| 纯度&分析 | Type II, aqueous glycerol solution, ≥35 units/mg protein |
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| 分子式 | N/A |
| 分子量 | N/A |
| 储存条件 | 2-8 °C 冷藏保存。 |
| 性质 |
| type |
Type II |
| form |
aqueous glycerol solution |
| shipped in |
wet ice |
| storage temp. |
2-8°C |
|
| 原产地 | 美国 |
| 描述 | Analysis Note
Protein determined by biuret.
Unit Definition
One unit will reduce 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.3 at 25 °C, in the presence of ammonium ions.
Physical form
Solution in 50% glycerol, pH 7.3
Application
L-glutamic dehydrogenase was used to catalyze the conversion of isocitrate into α-ketoglutarate and carbon dioxide.1
Biochem/physiol Actions
Mammalian forms of this enzyme, including this bovine form, can use either NADP(H) or NAD(H) as coenzymes. L-glutamic dehydrogenase plays a unique role in mammalian metabolism. The reverse reaction catalyzed by this enzyme is the only pathway by which ammonia can become bound to the α-carbon atom of an α-carboxylic acid and thus, is the only source of de novo amino acid synthesis in mammalian species.
The bovine enzyme is characterized by three sets of properties:
• It has a reversible concentration-dependent association, producing higher molecular weight forms.
• Forms tight enzyme-reduced coenzyme-substrate ternary complexes whose rates of dissociation modulate the steady-state reaction rates.
• Exhibits a wide variety of effects from the binding of any of a number of nucleotide modifiers.
L-glutamic dehydrogenase catalyzes the conversion of glutamate to α-ketoglutarate2,3.
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| 供货状态 | True |
| 参考文献 | 1. Glutamine supports pancreatic cancer growth through a KRAS-regulated metabolic pathway. Son J, Lyssiotis CA, Ying H, et al. Nature 496(7443), 101-5, (2013)
2. Glutamic dehydrogenase. STRECKER HJ Arch. Biochem. Biophys. 46(1), 128-40, (1953)
3. Regulation of bovine glutamate dehydrogenase. The effects of pH and ADP J. Bailey et al. J. Biol. Chem. 257, 5579-5583, (1982)
4.T he kinetic mechanism of ox liver glutamate dehydrogenase in the presence of the allosteric effector ADP D.P. Hornby et al. Biochem. J. 223, 161-168, (1984)
5. Mitochondrial targeting adaptation of the hominoid-specific glutamate dehydrogenase driven by positive Darwinian selection. Rosso L, Marques AC, Reichert AS, et al. PLoS Genet. 4(8), 1000150, (2008)
6. Spectrum of glutamate dehydrogenase mutations in Japanese patients with congenital hyperinsulinism and hyperammonemia syndrome. Aso K, Okano Y, Takeda T, et al. Osaka City Med. J. 57(1), 1-9, (2011)
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| 类型 | 酶 & 辅酶 |
| 安全信息 | Personal Protective Equipment：Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
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