中文名称英文名称CAS 编号规格所属品牌订货号
α-淀粉酶α-Amylase from porcine pancreasN/A500KUSIGMAA3176
α-淀粉酶α-Amylase from porcine pancreasN/A2.5MUSIGMAA3176
α-淀粉酶α-Amylase from porcine pancreasN/A10MUSIGMAA3176
品牌SIGMA
产品名称 α-淀粉酶 : α-Amylase from porcine pancreas ——【产品编号:SIGMA-A3176】
CAS编号N/A
别名β-N-acetylglucosaminidase porcine placenta,PPA,al,1,4 glucan-4-glucanohydrolase,porcine pancreas α-amylase,细菌性淀粉酶
级别科学级
纯度&分析Type VI-B, ≥10 units/mg solid
Tips来源于猪胰脏。
分子式N/A
分子量N/A
储存条件2-8 °C 冷藏保存。
性质
type Type VI-B
form (Obtained in ammonium sulfate solution2)
solubility H2O: soluble4 mg/mL (clear, colorless solution)
storage temp. 2-8°C
原产地美国
描述

包装

Package size based on α-amylase activity.

Quality

Contains lactose.

Unit Definition

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.

Biochem/physiol Actions

α-Amylase catalyzes the hydrolysis of a-(1,4) glycosidic linkages of starch components and glycogen. In mammals, α-amylase is present in both salivary and pancreatic secretions Porcine pancreatic α-amylase is an endo-type amylase. It catalyzes the hydrolysis of α-( 1,4) glucosidic bonds in amylose and amylopectin through multiple attack toward the nonreducing end. Two isoenzymes (PPA I and 11) are known for pig pancreatic α-amylase. They have the same molecular weight but differ slightly in amino acid composition and isoelectric point.1

General description

Molecular mass: 51-54 kDa.
α-Amylase isolated from porcine pancreas is a glycoprotein. It is a single polypeptide chain of ~475 residues containing two SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability. Chloride ions are necessary for activity and stability. The pH range for activity is 5.5 to 8.0, with the pH optimum at 7.

供货状态True
参考文献

1. Mixie Qian, Richard Haser, Carbohydrate binding sites in a pancreatic a-amylase-substrate complex, derived from X-ray structure analysis at 2.1 A resolution Protein Sci. 4, 747-755, (1995)

2. Cihangir Duy and Jo¨rg Fitter, Thermostability of Irreversible Unfolding Amylases Analyzed by Unfolding Kinetics J. Biol. Chem. 280, 37360-37365, (2005)

Pang, B.C., and Cheung, B.K., Applicability of two commercially available kits for forensic identification of saliva stains. J. Forensic Sci. 53, 1117-22, (2008)

类型酶 & 辅酶
安全信息
符号 GHS08
信号词 Danger
危险声明 H334
警示性声明 P261-P342 + P311
个人防护装备 dust mask type N95 (US), Eyeshields, Faceshields, Gloves
危害码 Xn
风险声明 42
安全声明 2-22-24-36/37
WGK德国 1
RTECS BU7432500