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中文名称英文名称CAS 编号规格目录价所属品牌订货号
吐温-20TWEEN® 209005-64-5100ML¥230.00SIGMA-ALDRICHP1379
吐温-20TWEEN® 209005-64-5500ML¥390.00SIGMA-ALDRICHP1379
品牌SIGMA-ALDRICH
产品名称 吐温-20 : TWEEN® 20 ——【产品编号:SIGMA-ALDRICH-P1379】
CAS编号9005-64-5
别名聚氧乙烯山梨糖醇酐单月桂酸酯,聚氧乙烯脱水山梨醇单月桂酸酯,吐温20
级别超纯级
纯度&分析viscous liquid
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分子式N/A
分子量N/A
储存条件常温避光保存。
性质

~Quality Level   PREMIUM
description   non-ionic
form   viscous liquid
mol wt   mol wt ~1228
composition   lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids)
impurities   ≤3.0% water
refractive index   n20/D 1.468(lit.)
CMC   0.06 mM(20-25°C)
acid number   ≤2.2 mg/g
hydroxyl value   96‑108 mg/g
transition temp   cloud point 76 °C
density   1.095 g/mL at 25 °C(lit.)
HLB   16.7
suitability   complies for IR spectroscopy

原产地美国
描述

~Application
非离子洗涤剂
TWEEN 20 has been used as a component of:
• washing buffer and blocking buffer in Western blotting
• blocking buffer in immunohistochemistry[1]
• washing buffer in ELISA (enzyme linked immunosorbent assay)[2][3]
• reaction mixture in PCR (polymerase chain reaction)[4]
TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.
Caution
TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions. Autoclaving is not recommended without testing for changes in properties. TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures. Polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid. They may also reduce the activity of many preservatives. Aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate.
包装
1, 4 L in poly bottle
25, 100, 250, 500, 6×500 mL in poly bottle
Preparation Note
TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.
General description
TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions.
法律信息
TWEEN 为以下机构的注册商标: Croda International PLC

供货状态True
参考文献N/A
类型BSZH 生物化学研究
安全信息

~个人防护装备  Eyeshields, Gloves
RIDADR  NONH for all modes of transport
WGK德国  1
RTECS  TR7400000
闪点(华氏)  >230 °F
闪点(摄氏)  >110 °C