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中文名称英文名称CAS 编号规格目录价所属品牌订货号
低熔点琼脂糖Agarose, low gelling temperature39346-81-15G¥517.00SIGMAA9414
低熔点琼脂糖Agarose, low gelling temperature39346-81-125G¥1,057.00SIGMAA9414
低熔点琼脂糖Agarose, low gelling temperature39346-81-1100G¥3,778.00SIGMAA9414
低熔点琼脂糖Agarose, low gelling temperature39346-81-11KG¥32,850.00SIGMAA9414
品牌SIGMA
产品名称 低熔点琼脂糖 : Agarose, low gelling temperature ——【产品编号:SIGMA-A9414】
CAS编号39346-81-1
别名2-羟乙基琼脂糖,球状琼脂糖,Agarose ME
级别电泳级
纯度&分析BioReagent, for molecular biology
Tips具有低凝胶温度的琼脂糖。
分子式N/A
分子量N/A
储存条件常温避光保存。
性质
grade for molecular biology
product line BioReagent
foreign activity DNase, RNase, NICKase, none detected
原产地美国
描述

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Application

本品经过改性,凝胶温度和熔点更低,便于从低熔点琼脂糖凝胶中回收和纯化 DNA。使用从低熔点琼脂糖凝胶上切下的 DNA 凝胶薄片可直接在重熔琼脂糖中制备放射性标记的 DNA 探针。
使用从低熔点琼脂糖凝胶上切下的 DNA 凝胶薄片可直接在重熔琼脂糖中方便地将限制性片段克隆到质粒中。
使用了溴化乙锭染料,因此凝胶具有低背景荧光。

供货状态True
参考文献

Sambrook, J., et al. Molecular Cloning: A Laboratory Manual 2nd ed., Plainview, NY , (1989), 6

Wieslander, L., A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels. Anal. Biochem. 98, 305, (1979)

Feinberg, A.P., Vogelstein, B., A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Addendum. Anal. Biochem. 137, 266, (1984)

Struhl, K. Biotechniques 3, 452, (1985)

类型分离试剂
安全信息
个人防护装备 Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK德国 3