中文名称英文名称CAS 编号规格所属品牌订货号
外源凝集素(PHA-L)Lectin from Phaseolus vulgaris (red kidney bean)N/A2MGSIGMAL2769
外源凝集素(PHA-L)Lectin from Phaseolus vulgaris (red kidney bean)N/A5MGSIGMAL2769
外源凝集素(PHA-L)Lectin from Phaseolus vulgaris (red kidney bean)N/A25MGSIGMAL2769
品牌SIGMA
产品名称 外源凝集素(PHA-L) : Lectin from Phaseolus vulgaris (red kidney bean) ——【产品编号:SIGMA-L2769】
CAS编号N/A
别名PHA,菜豆植物血球凝集素,植物血凝素,植物凝集素,植物外源凝集素,phytoh(a)emagglutinin,phytohemagglutinin,plant hemagglutinin
级别科学级
纯度&分析Leucoagglutinin PHA-L, lyophilized powder
TipsPHA,又名:植物血球凝集素。此产品为L型PHA,来源于菜豆。
分子式N/A
分子量N/A
储存条件2-8 °C 冷藏保存。
性质
form lyophilized powder
potency erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at 250 μg lectin/ml.)
  ≤1.0 μg/mL leucoagglutination activity
  ≤5 μg/mL mitogenic activity
composition Protein, ≥60% biuret
total impurities salt, free
storage temp. 2-8°C
原产地美国
描述

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of either human erythrocytes (2% in phosphate buffered saline, pH 6.8) or human leukocytes (107 per mL in saline) after 1 hr incubation at 25 °C.

Biochem/physiol Actions

PHA 由两个分子种类构成,一种是红细胞凝集素 (PHA-E),具有低促有丝分裂活性和高红细胞凝集活性,另一种是白细胞凝集素 (PHA-L),具有高促有丝分裂和白细胞凝集活性,但红细胞凝集活性极低。1,2
PHA-E 不具有血型特异性,但凝集作用受某些寡糖抑制。3PHA-P 是在分离和纯化红细胞凝集素和白细胞凝集素前 PHA 的蛋白质形式。4PHA-M 为粘蛋白形式。4由相应的已纯化凝集素制备偶联物。

Preparation Note

Purified by a modification of the method of Leavitt.5

供货状态True
参考文献

1. Yachnin, A. and Svenson, R.H. Immunology 22, 871, (1972)

2. Felsted, et al. J. Biol. Chem. 252, 2967, (1977)

3. Kornfeld, R. and Kornfeld, S. J. Biol. Chem. 245, 2536, (1970)

4. Rigas, D.A. and Osgood, E.E. J. Biol. Chem. 212, 607, (1955)

5. Leavitt, R.D., et al. J. Biol. Chem. 252, 2961, (1977)

Girnita, D.M., et al., Genotypic Variation And Phenotypic Characterization Of Granzyme B Gene Polymorphisms. Transplantation 87, 1801-6, (2009)

Lyke, K.E., et al., Cell-mediated Immunity Elicited By The Blood Stage Malaria Vaccine Apical Membrane Antigen 1 In Malian Adults: Results Of A Phase I Randomized Trial. Vaccine 27, 2171-6, (2009)

类型蛋白质
安全信息
个人防护装备 Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK德国 3