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中文名称英文名称CAS 编号规格目录价所属品牌订货号
BCIP5-Bromo-4-chloro-3-indolyl phosphate disodium salt102185-33-1100MG¥693.00SIGMAB6149
BCIP5-Bromo-4-chloro-3-indolyl phosphate disodium salt102185-33-1500MG¥2,259.00SIGMAB6149
BCIP5-Bromo-4-chloro-3-indolyl phosphate disodium salt102185-33-11G¥3,686.00SIGMAB6149
BCIP5-Bromo-4-chloro-3-indolyl phosphate disodium salt102185-33-15G¥14,780.00SIGMAB6149
品牌SIGMA
产品名称 BCIP : 5-Bromo-4-chloro-3-indolyl phosphate disodium salt ——【产品编号:SIGMA-B6149 】
CAS编号102185-33-1
别名5-溴-4-氯-3-吲哚基-磷酸盐,BCIP®,X-phosphate disodium salt
级别科学级
纯度&分析≥98% (HPLC)
Tips一种常用的生物染色剂,可用作生物染色、指示剂、底物等相关研究。
分子式 C8H4BrClNO4P · 2Na
分子量370.43
储存条件-20 °C 低温冷冻保存。
性质
assay   ≥98% (HPLC)
solubility   H2O: soluble20 mg/mL
  DMF: insoluble
storage temp.   −20°C
APPEARANCE WHITE TO LIGHT BLUE POWDER
SOLUBILITY CLEAR TO HAZY LIGHT YELLOW TO LIGHT BLUE SOLUTION AT 50 MG/ML IN WATER
WATER BY KARL FISCHER < OR = 10%
ELEMENTAL ANALYSIS * 25.0 TO 26.4% CARBON
  3.5 TO 4.1% NITROGEN
ICP-ATOMIC EMISSION * 12.0 TO 14.0% SODIUM
  7.8 TO 9.0% PHOSPHORUS
PURITY BY HPLC > OR = 98%
  * ANHYDROUS BASIS
原产地美国
描述

Substrates

Histochemical substrate for alkaline phosphatase.

Application

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.

BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

法律信息

BCIP is a registered trademark of Sigma-Aldrich Co. LLC

供货状态True
参考文献

McGadey, J., A tetrazolium method for non-specific alkaline phosphatase. Histochemie 23, 180-184, (1970)

Horwitz, J.P., et al. J. Med. Chem. 9, 447, (1966)

Leary, J.J., et al., Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots. Proc. Natl. Acad. Sci. U. S. A. 80, 4045-4049, (1983)

Knecht, D.A., Dimond, R.L., Visualization of antigenic proteins on Western blots. Anal. Biochem. 136, 180, (1984)

Walters, C., et al., Detection of parvovirus B19 in macerated fetal tissue using in situ hybridization. J. Clin. Pathol. 50(9), 749-754, (1997)

Meltzer, J.C., et al., Enhanced immunohistochemical detection of autonomic nerve fibers, cytokines and inducible nitric oxide synthase by light and fluorescent microscopy in rat spleen. J. Histochem. Cytochem. 45(4), 599-610, (1997)

类型染色剂
安全信息
个人防护装备  Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK德国  3