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中文名称英文名称CAS 编号规格目录价所属品牌订货号
透明质酸酶Hyaluronidase from bovine testes37326-33-330MG¥920.00SIGMAH4272
透明质酸酶Hyaluronidase from bovine testes37326-33-3100MG¥2,600.00SIGMAH4272
品牌SIGMA
产品名称 透明质酸酶 : Hyaluronidase from bovine testes ——【产品编号:SIGMA-H4272】
CAS编号37326-33-3
别名玻璃糖醛酸酶,玻璃酸酶,Hyaluronoglucosaminidase,Hyaluronate 4-glycanohydrolase
级别科学级
纯度&分析Type IV-S, powder, mouse embryo tested, 750-3000 units/mg solid
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分子式N/A
分子量N/A
储存条件-20 °C 低温冷冻保存。
性质

sterility    aseptically processed 
type    Type IV-S 
form    powder 
mol wt    mol wt ~55 kDa (four subunits of 14 kDa each) 
composition    Protein, 80-105% biuret 
suitability    mouse embryo tested 
storage temp.    −20°C 

原产地美国
描述

Biochem/physiol Actions

Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.

这些酶可随机裂解透明质酸、软骨素和硫酸软骨素中的 β-N-乙酰己糖胺-[1→4] 糖苷键。

Unit Definition

One unit will cause a change in A600 of 0.330 per minute at pH 5.35 at 37°C in a 2.0 ml reaction mixture (45 minute assay).

Application

Hyaluronidase is an enzyme derived from bovine testes. It is used to remove the cumulus mass from newly ovulated eggs, when preparing the eggs for in vitro culture.

Reconstitute 1 vial of hyaluronidase with 3 ml of M2 Medium (Product Nos. M 7167 or M 5910) or deionized water to obtain a 10 mg/ml stock solution. Dilute stock solution with M2 Medium to obtain a final working concentration of approximately 300 μg/ml.

Gonadotropins are used to superovulate 20 female mice. The mice are then mated and preimplantation stage embryos are retrieved and counted. The embryos are treated with hyaluronidase until the cumulus mass is dissociated from the embryos. The embryos are cultured, viewed daily and progress of the embryos is recorded.

供货状态True
参考文献N/A
类型酶 & 辅酶
安全信息

个人防护装备   Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter 
RIDADR   NONH for all modes of transport 
WGK德国   3