品牌 |  |
产品名称 |
外源凝集素(PHA-P) : Lectin from Phaseolus vulgaris (red kidney bean) ——【产品编号:SIGMA-L9017】
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CAS编号 | N/A |
别名 | PHA,菜豆植物血球凝集素,植物血凝素,植物凝集素,植物外源凝集素,phytoh(a)emagglutinin,phytohemagglutinin,plant hemagglutinin |
级别 | 科学级 |
纯度&分析 | Phytohemagglutinin PHA-P, BioXtra, purified by affinity chromatography, lyophilized powder |
Tips | PHA,又名:植物血球凝集素。此产品为P型PHA,来源于菜豆。 |
分子式 | N/A |
分子量 | N/A |
储存条件 | 2-8 °C 冷藏保存。 |
性质 |
product line |
BioXtra |
form |
lyophilized powder |
potency |
<16 μg per mL agglutination activity |
purified by |
affinity chromatography |
total impurities |
salt, essentially free |
storage temp. |
2-8°C |
|
原产地 | 美国 |
描述 |
Analysis Note
Agglutination activity is expressed in mg/mL and is determined from serial dilutions of a 1 mg/mL solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8 after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation.
Biochem/physiol Actions
PHA 由两个分子种类构成,一种是红细胞凝集素 (PHA-E),具有低促有丝分裂活性和高红细胞凝集活性,另一种是白细胞凝集素 (PHA-L),具有高促有丝分裂和白细胞凝集活性,但红细胞凝集活性极低。1,2
PHA-E 不具有血型特异性,但凝集作用受某些寡糖抑制。3PHA-P 是在分离和纯化红细胞凝集素和白细胞凝集素前 PHA 的蛋白质形式。4PHA-M 为粘蛋白形式。4由相应的已纯化凝集素制备偶联物。
Quality
Mitogenic activity at <10 μg per mL.
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供货状态 | True |
参考文献 |
1. Yachnin, A. and Svenson, R.H. Immunology 22, 871, (1972)
2. Felsted, et al. J. Biol. Chem. 252, 2967, (1977)
3. Kornfeld, R. and Kornfeld, S. J. Biol. Chem. 245, 2536, (1970)
4. Rigas, D.A. and Osgood, E.E. J. Biol. Chem. 212, 607, (1955)
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类型 | 蛋白质 |
安全信息 |
安全声明 |
22-24/25 |
WGK德国 |
3 |
RTECS |
TJ3470000 |
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